Mouse Anti-Goat IgG Fc-HRP

Cat. No.
6158-05  
Size
1.0 mL  
Price (USD)
$300.00 
Clone SB115d
Isotype Mouse (BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-HRP
Immunogen Pooled goat IgG
Specificity Goat IgG Fc
Minimal reactivity to goat IgG Fab and sheep, rabbit, human, monkey, rat, hamster, canine, and swine IgG
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested 1
FLISA – Quality tested
Western Blot – Reported in literature 2
Immunohistochemistry-Frozen Sections 3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796222
  • ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
  • ELISA plate was coated with purified mouse IgG Fc and IgG Fab and human, bovine, and horse serum. Immunoglobulins and sera were detected with serially diluted Goat Anti-Mouse IgG Fc, Human/Bovine/Horse SP ads-UNLB (SB Cat. No. 1013-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
  • ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ and mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa, Mouse ads-UNLB (SB Cat. No. 2061-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).

ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
 

ELISA plate was coated with purified mouse IgG Fc and IgG Fab and human, bovine, and horse serum. Immunoglobulins and sera were detected with serially diluted Goat Anti-Mouse IgG Fc, Human/Bovine/Horse SP ads-UNLB (SB Cat. No. 1013-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
 

ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ and mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa, Mouse ads-UNLB (SB Cat. No. 2061-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
 
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References
1. Tang J, Sun X, Yang H, Zhang B, Li Z, Lin Z, et al. Well-oriented ZZ-PS-tag with high Fc-binding onto polystyrene surface for controlled immobilization of capture antibodies. Anal Chim Acta. 2013;776:74-8. (ELISA)
2. Mohapatra A, Van Dyken SJ, Schneider C, Nussbaum JC, Liang H, Locksley RM. Group 2 innate lymphoid cells utilize the IRF4-IL-9 module to coordinate epithelial cell maintenance of lung homeostasis. Mucosal Immunol. 2016;9:275-86. (WB)
3. SouthernBiotech published data (IHC-FS)