Mouse Anti-Biotin-HRP

Cat. No.
6404-05  
Size
1.0 mL  
Price (USD)
$185.00 
Clone SB58c
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-HRP
Immunogen Biotin-KLH
Specificity Biotin
Description Anti-Biotin may be used for indirect immunostaining when working with a biotinylated primary antibody. As an effective alternative to avidin, Anti-Biotin amplifies signal and reduces background that can be caused by avidin and its derivatives.
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested
FLISA – Quality tested
Flow Cytometry – Quality tested
Western Blot – Reported in literature 1
Proximity Ligation Assay – Reported in literature 2
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796299
  • Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Erk1/2-BIOT (SB Cat. No. 12080-08). Proteins were visualized using Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05) secondary antibody and chemiluminescent detection.

Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Erk1/2-BIOT (SB Cat. No. 12080-08). Proteins were visualized using Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05) secondary antibody and chemiluminescent detection.
 
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References
1. Späte A, Schart VF, Häfner J, Niederwieser A, Mayer TU, Wittmann V. Expanding the scope of cyclopropene reporters for the detection of metabolically engineered glycoproteins by Diels-Alder reactions. Beilstein J Org Chem. 2014;10:2235-42. (WB)
2. Workman ER, Haddick PC, Bush K, Dilly GA, Niere F, Zemelman BV, et al. Rapid antidepressants stimulate the decoupling of GABAB receptors from GIRK/Kir3 channels through increased protein stability of 14-3-3η. Mol Psychiatry. 2015;20:298-310. (Proximity ligation assay)
3. Fahie MA, Yang B, Mullis M, Holden MA, Chen M. Selective detection of protein homologues in serum using an OmpG nanopore. Anal Chem. 2015;87:11143-9.