Mouse Anti-Chicken CD4-FITC

Cat. No.
8255-02  
Size
0.5 mg  
Price (USD)
$340.00 
Clone EP96
Isotype Mouse (BALB/c) IgMκ
Isotype Control Mouse IgM-FITC
Immunogen Chicken splenocytes
Specificity Chicken/Turkey CD4
Description CD4 is a type I transmembrane glycoprotein expressed on approximately 70% of thymocytes, 10% of spleen cells, and 45% of peripheral blood lymphocytes but less than 1% of bursal cells.
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 2-7
Immunoprecipitation 8
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796464
  • Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD4-FITC (SB Cat. No. 8255-02).

Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD4-FITC (SB Cat. No. 8255-02).
 
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References
1. Paramithiotis E, Tkalec L, Ratcliffe MJ. High levels of CD45 are coordinately expressed with CD4 and CD8 on avian thymocytes. J Immunol. 1991;147:3710-7. (Immunogen)
2. Marmor MD, Benatar T, Ratcliffe MJ. Retroviral transformation in vitro of chicken T cells expressing either α/β or γ/δ T cell receptors by reticuloendotheliosis virus strain T. J Exp Med. 1993;177:647-56. (FC)
3. Sayegh CE, Ratcliffe MJ. Perinatal deletion of B cells expressing surface Ig molecules that lack V(D)J-encoded determinants in the bursa of Fabricius is not due to intrafollicular competition. J Immunol. 2000;164:5041-8. (FC)
4. Yang X, Guo Y. Modulation of intestinal mucosal immunity by dietary polyunsaturated fatty acids in chickens. Food Agr Immunol. 2006;17:129-37. (FC)
5. Yang X, Guo Y, He X, Yuan J, Yang Y, Wang Z. Growth performance and immune responses in chickens after challenge with lipopolysaccharide and modulation by dietary different oils. Animal. 2008;2:216-23. (FC)
6. Yang X, Yao J, He X, Yang Y, Zhang B, Yuan J, et al. Dietary oils modulate T-cell differentiation and IL-2 bioactivity of intestinal mucosal lymphocytes in chickens. Food Agr Immunol. 2011;22:205-15. (FC)
7. Meyerhoff RR, Ali RA, Liu K, Huang G, Koci MD. Comprehensive analysis of commercially available mouse antichicken monoclonal antibodies for cross-reactivity with peripheral blood leukocytes from commercial turkeys. Poult Sci. 2012;91:383-92. (FC, Turkey Reactivity)
8. SouthernBiotech unpublished data (IP)