Mouse Anti-Human IgD-UNLB

Cat. No.
9030-01  
Size
0.5 mg  
Price (USD)
$125.00 
Clone IADB6
Isotype Mouse IgG2aκ
Isotype Control Mouse IgG2a-UNLB
Immunogen Human IgD myeloma protein
Specificity Human IgD
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested
FLISA – Quality tested
Flow Cytometry – Quality tested 4-10
Immunohistochemistry-Frozen Sections – Reported in literature 2
Immunohistochemistry-Paraffin Sections – Reported in literature 3
Immunoprecipitation – Reported in literature 1
Separation – Reported in literature 11,12
Costimulation – Reported in literature 13,14
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796590
Gene ID
3495 (Human)
Gene Symbol
IGHD (Human)
Uniprot ID
P01880 (Human)
Uniprot Name
  • ELISA plate was coated with purified human IgD, IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgD-UNLB (SB Cat. No. 9030-01) followed by Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05).

ELISA plate was coated with purified human IgD, IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgD-UNLB (SB Cat. No. 9030-01) followed by Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05).
 
Related Products (7)
References
1. Kiyotaki M, Cooper MD, Bertoli LF, Kearney JF, Kubagawa H. Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells. J Immunol. 1987;138:4150-8. (IP)
2. Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, et al. IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells. J Immunol. 2007;178:2872-82. (IHC-FS)
3. Shi S, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem. 1991;39:741-8. (IHC-PS)
4. Klein U, Küppers R, Rajewsky K. Variable region gene analysis of B cell subsets derived from a 4-year-old child: somatically mutated memory B cells accumulate in the peripheral blood already at young age. J Exp Med. 1994;180:1383-93. (FC)
5. Krzysiek R, Lefèvre EA, Zou W, Foussat A, Bernard J, Portier A, et al. Antigen receptor engagement selectively induces macrophage inflammatory protein-1α (MIP-1α) and MIP-1β chemokine production in human B cells. J Immunol. 1999;162:4455-63. (FC)
6. Jaimes MC, Rojas OL, Kunkel EJ, Lazarus NH, Soler D, Butcher EC, et al. Maturation and trafficking markers on rotavirus-specific B cells during acute infection and convalescence in children. J Virol. 2004;78:10967-76. (FC)
7. He B, Xu W, Santini PA, Polydorides AD, Chiu A, Estrella J, et al. Intestinal bacteria trigger T cell-independent immunoglobulin A2 class switching by inducing epithelial-cell secretion of the cytokine APRIL. Immunity. 2007;26:812-26. (FC)
8. Rojas OL, Caicedo L, Guzmán C, Rodríguez L, Castañeda J, Uribe L, et al. Evaluation of circulating intestinally committed memory B cells in children vaccinated with attenuated human rotavirus vaccine. Viral Immunol. 2007;20:300-11. (FC)
9. Smith K, Garman L, Wrammert J, Zheng N, Capra JD, Ahmed R, et al. Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen. Nature Protoc. 2009;4:372-84. (FC)
10. Suryani S, Fulcher DA, Santner-Nanan B, Nanan R, Wong M, Shaw PJ, et al. Differential expression of CD21 identifies developmentally and functionally distinct subsets of human transitional B cells. Blood. 2010;115:519-29. (FC)
11. Al-Darmaki S, Knightshead K, Ishihara Y, Best A, Schenkein HA, Tew JG, et al. Delineation of the role of platelet-activating factor in the immunoglobulin G2 antibody response. Clin Diagn Lab Immunol. 2004;11:720-8. (Sep)
12. Xu W, Santini PA, Matthews AJ, Chiu A, Plebani A, He B, et al. Viral double-stranded RNA triggers Ig class switching by activating upper respiratory mucosa B cells through an innate TLR3 pathway involving BAFF. J Immunol. 2008;181:276-87. (Sep)
13. Kuritani T, Cooper MD. Human B cell differentiation. III. Enhancing effect of monoclonal anti-immunoglobulin D antibody on pokeweed mitogen-induced plasma cell differentiation. J Immunol. 1982;129:2490-5. (Costim)
14. Igarashi H, Sakaguchi N. Telomerase activity is induced in human peripheral B lymphocytes by the stimulation to antigen receptor. Blood. 1997;89:1299-1307. (Costim)