Mouse Anti-Human CD16-FITC

Cat. No.
9571-02  
Size
100 tests  
Price (USD)
$190.00 
Clone 3G8
Isotype Mouse (CD2F1) IgG1κ
Isotype Control Mouse IgG1-FITC
Immunogen Human polymorphonuclear leukocytes
Specificity Human/Chimpanzee CD16
Alternalte Name(s) FcγRIII, low affinity Fc receptor
Description CD16, a member of the immunoglobulin superfamily, is a 50-65 kDa glycoprotein found as both a transmembrane and GPI-linked form. The transmembrane form of CD16 is expressed on NK cells, granulocytes, macrophages, and mast cells but not on eosinophils. The GPI-anchored type of CD16 is found only on neutrophils. CD16 is involved in NK activation and signal transduction.
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 2,3,5-7
Immunohistochemistry-Frozen Sections – Reported in literature 4
Immunocytochemistry – Reported in literature 3
Immunoprecipitation – Reported in literature 1,2
Western Blot-Non-Reducing – Reported in literature 3
Stimulation – Reported in literature 5
Costimulation – Reported in literature 5
Blocking – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796962
Gene ID
2214 (Human)
2215 (Human)
457457 (Chimpanzee)
745350 (Chimpanzee)
Gene Symbol
FCGR3A (Human)
FCGR3B (Human)
LOC457457 (Chimpanzee)
LOC745350 (Chimpanzee)
Gene Aliases CD16; CD16A; FCG3; FCGR3; FCGRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20; CD16b; FCGR3A; FCRIIIb; CK820_G0056287; FCGR3B
Uniprot ID
P08637 (Human)
O75015 (Human)
Uniprot Name
  • Human peripheral blood granulocytes were stained with Mouse Anti-Human CD16-PACBLU (SB Cat. No. 9571-26).

Human peripheral blood granulocytes were stained with Mouse Anti-Human CD16-PACBLU (SB Cat. No. 9571-26).
 
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References
1. Fleit HB, Wright SD, Unkeless JC. Human neutrophil Fcγ receptor distribution and structure. Proc Natl Acad Sci USA. 1982;79:3275-9. (Immunogen, Block, IP)
2. Edberg JC, Kimberly RP. Cell type-specific glycoforms of FcγRIIIa (CD16) differential ligand binding. J Immunol. 1997;159:3849-57. (FC, IP)
3. Tosi MF, Zakem H. Surface expression of Fcγ receptor III (CD16) on chemoattractant-stimulated neutrophils is determined by both surface shedding and translocation from intracellular storage compartments. J Clin Invest. 1992;90:462-70. (FC, ICC, WB)
4. Sedmak DD, Davis DH, Singh U, van de Winkel JG, Anderson CL. Expression of IgG Fc receptor antigens in placenta and on endothelial cells in humans. An immunohistochemical study. Am J Pathol. 1991;138:175-81. (IHC-FS)
5. Hoshino S, Oshimi K, Teramura M, Mizoguchi H. Activation via the CD3 and CD16 pathway mediates interleukin-2-dependent autocrine proliferation of granular lymphocytes in patients with granular lymphocyte proliferative disorders. Blood. 1991;78:3232-40. (FC, Stim, Costim)
6. Mavoungou E, Bouyou-Akotet MK, Kremsner PG. Effects of prolactin and cortisol on natural killer (NK) cell surface expression and function of human natural cytotoxicity receptors (NKp46, NKp44 and NKp30). Clin Exp Immunol. 2004;139:287-96. (FC)
7. Reeves RK, Evans TI, Fultz PN, Johnson RP. Potential confusion of contaminating CD16+ myeloid DCs with anergic CD16+ NK cells in chimpanzees. Eur J Immunol. 2011;41:1070-4. (FC, Chimpanzee Reactivity)