Mouse Anti-Human CD23-PE

Cat. No.
9580-09  
Size
100 tests  
Price (USD)
$218.00 
Clone Tü1
Isotype Mouse IgG3κ
Isotype Control Mouse IgG3-PE
Immunogen KR3598 cells
Specificity Human CD23
Alternalte Name(s) FCεRII, B6, Leu-20, BLAST-2, low affinity IgE receptor
Description CD23 is a 45 kDa type II integral membrane glycoprotein that is the low affinity receptor for IgE. It also binds to CD21 and the α chains of CD11b and CD11c. It is expressed by B cells and monocytes and, weakly, on T cells, follicular dendritic cells, eosinophils, NK cells, Langerhans cells, and platelets. It is upregulated in response to B cell activation, CD40 ligation, or in response to IL-4 or IL-13. CD23 is responsible for regulation of IgE synthesis and may play a role in antigen presentation by B cells and follicular dendritic cells by interacting with CD40.
Format/Conjugate PE (R-phycoerythrin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 2-5
Immunohistochemistry-Frozen Sections – Reported in literature 1,6,7
Immunocytochemistry – Reported in literature 7,8
Western Blot – Reported in literature 7,9,10
Blocking – Reported in literature 7
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796973
  • Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD23-PE (SB Cat. No. 9580-09) and Mouse Anti-Human CD3-FITC (SB Cat. No. 9515-02).

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD23-PE (SB Cat. No. 9580-09) and Mouse Anti-Human CD3-FITC (SB Cat. No. 9515-02).
 
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References
1. Ziegler A, Stein H, Müller C, Wernet P. Tü1: a monoclonal antibody defining a B cell subpopulation - usefulness for the classificaton of non-Hodgkin's lymphomas. In: Knapp W, editor. Leukemia Markers: Proceedings of the Leukemia Marker Conference held in Vienna, February 15-18, 1981. London: Academic Press; 1981. p. 113-6. (Immunogen, IHC-FS)
2. Sallusto F, Cella M, Danieli C, Lanzavecchia A. Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartment: downregulation by cytokines and bacterial products. J Exp Med. 1995;182:389-400. (FC)
3. Del Giudice I, Matutes E, Morilla R, Morilla A, Owusu-Ankomah K, Rafiq F, et al. The diagnostic value of CD123 in B-cell disorders with hairy or villous lymphocytes. Haematologica. 2004;89:303-8. (FC)
4. Johnson SE, Shah N, Panoskaltsis-Mortari A, LeBien TW. Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells. J Immunol. 2005;175:7325-31. (FC)
5. Vaisitti T, Gaudino F, Ouk S, Moscvin M, Vitale N, Serra S, et al. Targeting metabolism and survival in chronic lymphocytic leukemia and Richter syndrome cells by a novel NF-κB inhibitor. Haematologica. 2017;102:1878-89. (FC)
6. Hermes B, Worm M, Nowak F, Kroczek RA, Stein H, Henz BM. Upregulation of CD40 and CD40 ligand expression in IgE-associated cutaneous diseases. Acta Derm Venereol. 1997;77:441-5. (IHC-FS)
7. Tu Y, Salim S, Bourgeois J, Di Leo V, Irvine EJ, Marshall JK, et al. CD23-mediated IgE transport across human intestinal epithelium: inhibition by blocking sites of translation or binding. Gastroenterology. 2005;129:928-40. (IHC-FS, ICC, WB, Block)
8. Mason DY, Comans-Bitter WM, Cordell JL, Verhoeven MJ, van Dongen JJ. Antibody L26 recognizes an intracellular epitope on the B-cell-associated CD20 antigen. Am J Pathol. 1990;136:1215-22. (ICC)
9. Tu Y, Perdue MH. CD23-mediated transport of IgE/immune complexes across human intestinal epithelium: role of p38 MAPK. Am J Physiol Gastrointest Liver Physiol. 2006;291:G532-8. (WB)
10. Tu Y, Oluwole C, Struiksma S, Perdue MH, Yang PC. Mast cells modulate transport of CD23/IgE/antigen complex across human intestinal epithelial barrier. N Am J Med Sci. 2009;1:16-24. (WB)