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ApoScreen® Annexin V Apoptosis Kit-FITC

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100 tests  
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Description ApoScreen® Annexin V Apoptosis Kit-FITC (SB Cat. No. 10010-02) employs a fluorescein-conjugated Annexin V (Annexin V-FITC) in concert with propidium iodide (PI) to evaluate subpopulations of cells undergoing apoptosis. During the early stages of apoptosis, cells begin to display phosphatidylserine (PS) on the outer cell membrane where it is readily detectable by staining the cells with Annexin V-FITC. As the plasma membrane becomes increasingly permeable during the later stages of apoptosis, PI can readily move across the cell membrane and bind to cellular DNA, providing a means for identifying those cells that have lost membrane integrity through mechanisms including necrosis. When cells are double stained with Annexin V-FITC and PI, three different cell populations may be observed - (i) live cells that do not stain with either Annexin V-FITC or PI; (ii) necrotic cells that stain with both reagents; and (iii) apoptotic cells that stain with Annexin V-FITC only. Analysis may be performed on any flow cytometer equipped with a single laser using excitation at 488 nm. Alternatively, the cells can be examined with a fluorescence microscope equipped with filters for discrimination of FITC and rhodamine/PI.
Kit Components Annexin V-FITC – 100 tests in 1.0 mL
Propidium Iodide (PI) – 100 tests in 1.0 mL
Annexin V Binding Buffer 10X – 7 x 1.5 mL
Buffer Formulation Annexin V-FITC and PI are supplied in Annexin V Binding Buffer
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications Flow Cytometry – Quality tested 1-7, 9-34
Immunocytochemistry – Reported in literature 6,8,35
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Day old BALB/c mouse splenocytes were stained with ApoScreen® Annexin V Apoptosis Kit-FITC (SB Cat. No. 10010-02).

Day old BALB/c mouse splenocytes were stained with ApoScreen® Annexin V Apoptosis Kit-FITC (SB Cat. No. 10010-02).
1. Silkov A, Wolstein O, Shachar I, Dikstein R. Enhanced apoptosis of B and T lymphocytes in TAFII105 dominant-negative transgenic mice is linked to nuclear factor-κB. J Biol Chem. 2002;277:17821-9. (FC)
2. Catalan MP, Esteban J, Subirá D, Egido J, Ortiz A. Inhibition of caspases improves bacterial clearance in experimental peritonitis. Perit Dial Int. 2003;23:123-6. (FC)
3. Lester EA, Babensee JE. Proinflammatory phenotype of endothelial cells after coculture with biomaterial-treated blood cells. J Biomed Mater Res A. 2003;64:397-410. (FC)
4. Shiner EK, Terentyev D, Bryan A, Sennoune S, Martinez-Zaguilan R, Li G, et al. Pseudomonas aeruginosa autoinducer modulates host cell responses through calcium signalling. Cell Microbiol. 2006;8:1601-10. (FC)
5. Kikuno N, Shiina H, Urakami S, Kawamoto K, Hirata H, Tanaka Y, et al. Knockdown of astrocyte-elevated gene-1 inhibits prostate cancer progression through upregulation of FOXO3a activity. Oncogene. 2007;26:7647-55. (FC)
6. Petreaca ML, Yao M, Ware C, Martins-Green MM. Vascular endothelial growth factor promotes macrophage apoptosis through stimulation of tumor necrosis factor superfamily member 14 (TNFSF14/LIGHT). Wound Repair Regen. 2008;16:602-14. (FC, ICC)
7. Rong S, Cai JH, Andrews J. Cloning and apoptosis-inducing activities of canine and feline TRAIL. Mol Cancer Ther. 2008;7:2181-91. (FC)
8. Lai JC, Lai MB, Jandhyam S, Dukhande VV, Bhushan A, Daniels CK, et al. Exposure to titanium dioxide and other metallic oxide nanoparticles induces cytotoxicity on human neural cells and fibroblasts. Int J Nanomedicine. 2008;3:533-45. (ICC)
9. Rodrigues DH, Vilela MC, Barcelos LS, Pinho V, Teixeira MM, Teixeira AL. Absence of PI3Kγ leads to increased leukocyte apoptosis and diminished severity of experimental autoimmune encephalomyelitis. J Neuroimmunol. 2010;222:90-4. (FC)
10. Grishko V, Xu M, Wilson G, Pearsall AW. Apoptosis and mitochondrial dysfunction in human chondrocytes following exposure to lidocaine, bupivacaine, and ropivacaine. J Bone Joint Surg Am. 2010;92:609-18. (FC)
11. Ramaswamy S, Schornack PA, Smelko AG, Boronyak SM, Ivanova J, Mayer JE Jr, et al. Superparamagnetic iron oxide (SPIO) labeling efficiency and subsequent MRI tracking of native cell populations pertinent to pulmonary heart valve tissue engineering studies. NMR Biomed. 2012;25:410-7. (FC)
12. Kim J, Xu M, Xo R, Mates A, Wilson GL, Pearsall AW 4th, et al. Mitochondrial DNA damage is involved in apoptosis caused by pro-inflammatory cytokines in human OA chondrocytes. Osteoarthritis Cartilage. 2010;18:424-32. (FC)
13. Wang S, Tang Y, Cui H, Zhao X, Luo X, Pan W, et al. Let-7/miR-98 regulate Fas and Fas-mediated apoptosis. Genes Immun. 2011;12:149-54. (FC)
14. Hagner PR, Mazan-Mamczarz K, Dai B, Bolzer EM, Corl S, Martin SS, et al. Ribosomal protein S6 is highly expressed in non-Hodgkin lymphoma and associates with mRNA containing a 5' terminal oligopyrimidine tract. Oncogene. 2011;30:1531-41. (FC)
15. Matsuo AL, Figueiredo CR, Arruda DC, Pereira FV, Scutti JA, Massaoka MH, et al. α-Pinene isolated from Schinus terebinthifolius Raddi (Anacardiaceae) induces apoptosis and confers antimetastatic protection in a melanoma model. Biochem Biophys Res Commun. 2011;411:449-54. (FC)
16. Kawas LH, Yamamoto BJ, Wright JW, Harding JW. Mimics of the dimerization domain of hepatocyte growth factor exhibit anti-Met and anticancer activity. J Pharmacol Exp Ther. 2011;339:509-18. (FC)
17. Li W, Ma M, Dong L, Wang F, Chen L, Li X. MicroRNA-34a targets notch1 and inhibits cell proliferation in glioblastoma multiforme. Cancer Biol Ther. 2011;12:477-83. (FC)
18. Erfe MC, David CV, Huang C, Lu V, Maretti-Mira AC, Haskell J, et al. Efficacy of synthetic peptides RP-1 and AA-RP-1 against Leishmania species in vitro and in vivo. Antimicrob Agents Chemother. 2012;56:658-65. (FC)
19. Al-Owais MM, Scragg JL, Dallas ML, Boycott HE, Warburton P, Chakrabarty A, et al. Carbon monoxide mediates the anti-apoptotic effects of heme oxygenase-1 in medulloblastoma DAOY cells via K+ channel inhibition. J Biol Chem. 2012;287:24754-64. (FC)
20. Jackson LJ, Pheneger JA, Pheneger TJ, Davis G, Wright AD, Robinson JE, et al. The role of PIM kinases in human and mouse CD4+ T cell activation and inflammatory bowel disease. Cell Immunol. 2012;272:200-13. (FC)
21. Sanchez-Niño MD, Sanz AB, Sanchez-Lopez E, Ruiz-Ortega M, Benito-Martin A, Saleem MA, et al. HSP27/HSPB1 as an adaptive podocyte antiapoptotic protein activated by high glucose and angiotensin II. Lab Invest. 2012;92:32-45. (FC)
22. Polotskaia A, Hoffman S, Krett NL, Shanmugam M, Rosen ST, Bargonetti J. 8-Amino-adenosine activates p53-independent cell death of metastatic breast cancers. Mol Cancer Ther. 2012;11:2495-504. (FC)
23. Kushiro K, Núñez NP. Ethanol inhibits B16-BL6 melanoma metastasis and cell phenotypes associated with metastasis. In Vivo. 2012;26:47-58. (FC)
24. Babcock AS, Anderson AE, Rice CD. Indirubin-3'-(2,3 dihydroxypropyl)-oximether (E804) is a potent modulator of LPS-stimulated macrophage functions. Toxicol Appl Pharmacol. 2013;266:157-66. (FC)
25. Wang W, Li Q, Zou F, Yu Z, Wang Y, Lu T, et al. Increased expression of AGS3 in rat brain cortex after traumatic brain injury. J Neurosci Res. 2013;91:726-36. (FC)
26. Shimoni S, Bar I, Zilberman L, Goland S, Edri O, Gandelman G, et al. Circulating progenitor and apoptotic progenitor cells in patients with aortic regurgitation. Circ J. 2013;77:764-71. (FC)
27. Bailey ST, Miron PL, Choi YJ, Kochupurakkal B, Maulik G, Rodig SJ, et al. NF-κB activation-induced anti-apoptosis renders HER2-positive cells drug resistant and accelerates tumor growth. Mol Cancer Res. 2014;12:408-20. (FC)
28. Armstead AL, Arena CB, Li B. Exploring the potential role of tungsten carbide cobalt (WC-Co) nanoparticle internalization in observed toxicity toward lung epithelial cells in vitro. Toxicol Appl Pharmacol. 2014;278:1-8. (FC)
29. Niedzwiedz A, Jaworski Z, Tykalowski B, Smialek M. Neutrophil and macrophage apoptosis in bronchoalveolar lavage fluid from healthy horses and horses with recurrent airway obstruction (RAO). BMC Vet Res. 2014;10:29. (FC)
30. Razi SS, Rehmani S, Li X, Park K, Schwartz GS, Latif MJ, et al. Antitumor activity of paclitaxel is significantly enhanced by a novel proapoptotic agent in non-small cell lung cancer. J Surg Res. 2015;194:622-30. (FC)
31. Wang Y, Wu Y, Miao X, Zhu X, Miao X, He Y, et al. Silencing of DYRK2 increases cell proliferation but reverses CAM-DR in Non-Hodgkin's Lymphoma. Int J Biol Macromol. 2015;81:809-17. (FC)
32. Ko B, Lee S, Lee Y, Hwang I, Persson H, Rockberg J, et al. Combination of novel HER2-targeting antibody 1E11 with trastuzumab shows synergistic antitumor activity in HER2-positive gastric cancer. Mol Oncol. 2015;9:398-408. (FC)
33. Delma CR, Somasundaram ST, Srinivasan GP, Khursheed M, Bashyam MD, Aravindan N. Fucoidan from Turbinaria conoides: a multifaceted 'deliverable' to combat pancreatic cancer progression. Int J Biol Macromol. 2015;74:447-57 (FC)
34. Zhang D, Wang L, Yan L, Miao X, Gong C, Xiao M, et al. Vacuolar protein sorting 4B regulates apoptosis of intestinal epithelial cells via p38 MAPK in Crohn's disease. Exp Mol Pathol. 2015;98:55-64. (FC)
35. Figueiredo CR, Matsuo AL, Pereira FV, Rabaça AN, Farias CF, Girola N, et al. Pyrostegia venusta heptane extract containing saturated aliphatic hydrocarbons induces apoptosis on B16F10-Nex2 melanoma cells and displays antitumor activity in vivo. Pharmacogn Mag. 2014;10:S363-76. (ICC)
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