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ApoScreen® Annexin V Apoptosis Kit-PE

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100 tests  
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Description ApoScreen® Annexin V Apoptosis Kit-PE (SB Cat. No. 10010-09) employs a R-phycoerythrin-conjugated Annexin V (Annexin V-PE) in concert with 7-Aminoactinomycin D (7-AAD) to evaluate subpopulations of cells undergoing apoptosis. During the early stages of apoptosis, cells begin to display phosphatidylserine (PS) on the outer cell membrane where it is readily detectable by staining the cells with Annexin V-PE. As the plasma membrane becomes increasingly permeable during the later stages of apoptosis, 7-AAD can readily move across the cell membrane and bind to cellular DNA, providing a means for identifying those cells that have lost membrane integrity through mechanisms including necrosis. When cells are double stained with Annexin V-PE and 7-AAD, three different cell populations may be observed - (i) live cells that do not stain with either Annexin V-PE or 7-AAD; (ii) necrotic cells that stain with both reagents; and (iii) apoptotic cells that stain with Annexin V-PE only. Analysis may be performed on any flow cytometer equipped with a single laser using excitation at 488 nm.
Kit Components Annexin V-PE – 100 tests in 1.0 mL
7-Aminoactinomycin D (7-AAD) – 100 tests in 1.0 mL
Annexin V Binding Buffer 10X – 7 x 1.5 mL
Buffer Formulation Annexin V-PE is supplied in PBS/NaN3 and a stabilizing agent
7-AAD is supplied in PBS/NaN3
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications Flow Cytometry – Quality tested 1-35
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Day old BALB/c mouse splenocytes were stained with ApoScreen® Annexin V Apoptosis Kit-PE (SB Cat. No. 10010-09).

Day old BALB/c mouse splenocytes were stained with ApoScreen® Annexin V Apoptosis Kit-PE (SB Cat. No. 10010-09).
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ApoScreen® is a registered trademark of SouthernBiotechnology Associates, Inc.
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