Mouse Type IV Collagen-Solution

Cat. No.
1250-04S  
Size
0.5 mg  
Price (USD)
$200.00 
Description Collagen is the main structural protein in the extracellular space and is the most abundant protein in the ECM. Collagens are divided into two classes - fibril (types I, II, III, V) and non-fibril (types IV, VI). Type IV collagen is primarily expressed in the basal lamina and therefore constitutes a portion of the basement membrane. Type IV collagen mutations are associated with Alport syndrome and the α3(IV) chain is thought to be the antigen implicated in Goodpasture's syndrome. Type IV collagen is comprised of six isomeric chains designated α1(IV) to α6(IV).
Source Engelbreth-Holm-Swarm (EHS) sarcoma
Purity > 90% by SDS-PAGE
Purification Controlled and limited pepsin digestion followed by selective salt precipitation
Alternalte Name(s) Col4a1, Col4a2, Col4a3, Col4a4, Col4a5, Col4a6
Buffer Formulation 500 mM acetic acid
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications ELISA – Quality tested
SDS-PAGE – Quality tested
Microarray – Reported in literature 1
Cell Culture – Reported in literature 1
Coating Material for –
Adhesion Studies – Reported in literature 2
Aggregation Studies – Reported in literature 2
Blood Disorder Studies – Reported in literature 2
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with serially diluted Mouse Type IV Collagen (SB Cat. No. 1250-04S). Purified collagen was detected with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08), Goat Anti-Type II Collagen-BIOT (SB Cat. No. 1320-08), Goat Anti-Type III Collagen-BIOT (SB Cat. No. 1330-08), Goat Anti-Type IV Collagen-BIOT (SB Cat. No. 1340-08), Goat Anti-Type V Collagen-BIOT (SB Cat. No. 1350-08), and Goat Anti-Type VI Collagen-BIOT (SB Cat. No. 1360-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

ELISA plate was coated with serially diluted Mouse Type IV Collagen (SB Cat. No. 1250-04S). Purified collagen was detected with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08), Goat Anti-Type II Collagen-BIOT (SB Cat. No. 1320-08), Goat Anti-Type III Collagen-BIOT (SB Cat. No. 1330-08), Goat Anti-Type IV Collagen-BIOT (SB Cat. No. 1340-08), Goat Anti-Type V Collagen-BIOT (SB Cat. No. 1350-08), and Goat Anti-Type VI Collagen-BIOT (SB Cat. No. 1360-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 
Related Products (4)
References
1. Hou L, Coller J, Natu V, Hastie TJ, Huang NF. Combinatorial extracellular matrix microenvironments promote survival and phenotype of human induced pluripotent stem cell-derived endothelial cells in hypoxia. Acta Biomater. 2016;44:188-99. (Microarray, Cell Culture)
2. Slobodianuk TL, Kochelek C, Foeckler J, Kalloway S, Weiler H, Flood VH. Defective collagen binding and increased bleeding in a murine model of von Willebrand disease affecting collagen IV binding. J Thromb Haemost. 2019;17:63-71. (ELISA, Coating, Blood Disorder Studies, Adhesion Studies, Aggregation Studies)