Gelatin Subbed Slides

Cat. No.
SLD01-BX  
Size
72 Slides/Box  
Price (USD)
$60.00 
Description SouthernBiotech’s Gelatin Subbed Slides have been carefully cleaned and subbed with a solution of 0.1% gelatin and chromium potassium sulfate. The coated slides measure 25 mm x 75 mm with a frosted end for marking. For optimal adhesion, we recommend sections less than 100 μm in thickness.
Storage & Handling Store at -80°C
Please refer to product specific SDS
Applications Frozen Sections 3,6
Free Floating Frozen Sections 4,5,7,8
Resin Embedded Sections 2
TUNEL 1
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Free-floating Bcs1lc.232A>G mouse cerebral cortex cryosection was stained with anti-GFAP followed by secondary reagents, DAB, and mounted on Gelatin Subbed Slides (SB Cat. No. SLD01).

    Image from Tegelberg S, Tomašić N, Kallijärvi J, Purhonen J, Elmér E, Lindberg E, et al. Respiratory chain complex III deficiency due to mutated BCS1L: a novel phenotype with encephalomyopathy, partially phenocopied in a Bcs1l mutant mouse model. Orphanet J Rare Dis. 2017;12:73. Figure 6(a)
  • Free-floating Bcs1lc.232A>G mouse cerebral cortex cryosection was stained with anti-GFAP followed by secondary reagents, DAB, and mounted on Gelatin Subbed Slides (SB Cat. No. SLD01).

    Image from Tegelberg S, Tomašić N, Kallijärvi J, Purhonen J, Elmér E, Lindberg E, et al. Respiratory chain complex III deficiency due to mutated BCS1L: a novel phenotype with encephalomyopathy, partially phenocopied in a Bcs1l mutant mouse model. Orphanet J Rare Dis. 2017;12:73. Figure 6(c)

Free-floating Bcs1lc.232A>G mouse cerebral cortex cryosection was stained with anti-GFAP followed by secondary reagents, DAB, and mounted on Gelatin Subbed Slides (SB Cat. No. SLD01).

Image from Tegelberg S, Tomašić N, Kallijärvi J, Purhonen J, Elmér E, Lindberg E, et al. Respiratory chain complex III deficiency due to mutated BCS1L: a novel phenotype with encephalomyopathy, partially phenocopied in a Bcs1l mutant mouse model. Orphanet J Rare Dis. 2017;12:73. Figure 6(a)
 

Free-floating Bcs1lc.232A>G mouse cerebral cortex cryosection was stained with anti-GFAP followed by secondary reagents, DAB, and mounted on Gelatin Subbed Slides (SB Cat. No. SLD01).

Image from Tegelberg S, Tomašić N, Kallijärvi J, Purhonen J, Elmér E, Lindberg E, et al. Respiratory chain complex III deficiency due to mutated BCS1L: a novel phenotype with encephalomyopathy, partially phenocopied in a Bcs1l mutant mouse model. Orphanet J Rare Dis. 2017;12:73. Figure 6(c)
 
References
1. Lee T, Zhang X, Dhar S, Faas H, Lippard SJ, Jasanoff A. In vivo imaging with a cell-permeable porphyrin-based MRI contrast agent. Chem Biol. 2010;17:665-73. (TUNEL)
2. Curcio CA, Messinger JD, Sloan KR, Mitra A, McGwin G, Spaide RF. Human chorioretinal layer thicknesses measured in macula-wide, high-resolution histologic sections. Invest Ophthalmol Vis Sci. 2011;52:3943-54. (Resin embedded sections)
3. Deane EC, Ilie AE, Sizdahkhani S, Gupta MD, Orlowski J, McKinney RA. Enhanced recruitment of endosomal Na+/H+ exchanger NHE6 into dendritic spines of hippocampal pyramidal neurons during NMDA receptor-dependent long-term potentiation. J Neurosci. 2013;33:595-610. (Frozen sections)
4. Hodor A, Palchykova S, Baracchi F, Noain D, Bassetti CL. Baclofen facilitates sleep, neuroplasticity, and recovery after stroke in rats. Ann Clin Transl Neurol. 2014;1:765-77. (Free floating sections)
5. Kim KY, Scholl ES, Liu X, Shepherd A, Haeseleer F, Lee A. Localization and expression of CaBP1/caldendrin in the mouse brain. Neuroscience. 2014;268:33-47. (Free floating sections)
6. Adjei S, Wesson DW. Laminar and spatial localization of the islands of Calleja in mice. Neuroscience. 2015;287:137-43. (Frozen sections)
7. Tegelberg S, Tomašić N, Kallijärvi J, Purhonen J, Elmér E, Lindberg E, et al. Respiratory chain complex III deficiency due to mutated BCS1L: a novel phenotype with encephalomyopathy, partially phenocopied in a Bcs1l mutant mouse model. Orphanet J Rare Dis. 2017;12:73. (Free floating frozen sections)
8. Morabito MV, Ravussin Y, Mueller BR, Skowronski AA, Watanabe K, Foo KS, et al. Weight perturbation alters leptin signal transduction in a region-specific manner throughout the brain. PLoS One. 2017;12(1): e0168226. (Free floating frozen sections)