Human IgG1 Lambda-AP

Cat. No.
0151L-04  
Size
1.0 mL  
Price (USD)
$140.00 
Source Human myeloma serum
Format/Conjugate AP (Alkaline Phosphatase)
Buffer Formulation 50 mM Tris/1 mM MgCl2/50% Glycerol containing 0.1% sodium azide, pH 8.0
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1,2
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with serially diluted Human IgG1 Lambda-UNLB (SB Cat. No. 0151L-01). Immunoglobulin was detected with Mouse Anti-Human IgG1 Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG2 Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG3 Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG4 pFc’-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA1-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA2-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.

ELISA plate was coated with serially diluted Human IgG1 Lambda-UNLB (SB Cat. No. 0151L-01). Immunoglobulin was detected with Mouse Anti-Human IgG1 Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG2 Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG3 Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG4 pFc’-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA1-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA2-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
 
References
1. Zhang J, Jacobi AM, Mackay M, Aranow C, Wang T, Chinnasamy P, et al. Identification of DNA-reactive B cells in patients with systemic lupus erythematosus. J Immunol Methods. 2008;338:79-84. (ELISA)
2. Zhang J, Jacobi AM, Wang T, Berlin R, Volpe BT, Diamond B. Polyreactive autoantibodies in systemic lupus erythematosus have pathogenic potential. J Autoimmun. 2009;33:270-4. (ELISA)
3. Liu H, Chumsae C, Gaza-Bulseco G, Hurkmans K, Radziejewski CH. Ranking the susceptibility of disulfide bonds in human IgG1 antibodies by reduction, differential alkylation, and LC-MS analysis. Anal Chem. 2010;82:5219-26. (Mass spectrometry)