Goat Anti-Mouse IgG3, Human ads-FITC

Cat. No.
1100-02  
Size
1.0 mg  
Price (USD)
$195.00 
Specificity Reacts with the heavy chain of mouse IgG3
Source Pooled antisera from goats hyperimmunized with mouse IgG3
Cross Adsorption Mouse IgG1, IgG2a, IgG2b, IgM, and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgG3 covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-FITC
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-9
FLISA 2
Flow Cytometry 10-16
Other referenced applications for relevant formats include -
ELISpot 1,3,4,10
Immunohistochemistry-Frozen Sections 17-21
Immunohistochemistry-Paraffin Sections 17,22
Immunocytochemistry 23
Western Blot 1,24
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794570
  • FLISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG3, Human ads-FITC (SB Cat. No. 1100-02).
  • Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD23-UNLB (SB Cat. No. 9580-01) and Mouse Anti-Human CD3-PE (SB Cat. No. 9515-09) followed by Goat Anti-Mouse IgG3, Human ads-FITC (SB Cat. No. 1100-02).

FLISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG3, Human ads-FITC (SB Cat. No. 1100-02).
 

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD23-UNLB (SB Cat. No. 9580-01) and Mouse Anti-Human CD3-PE (SB Cat. No. 9515-09) followed by Goat Anti-Mouse IgG3, Human ads-FITC (SB Cat. No. 1100-02).
 
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References
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