Goat Anti-Type I Collagen-UNLB

Cat. No.
1310-01  
Size
0.2 mg  
Price (USD)
$280.00 
Specificity Reacts with conformational determinants on type I collagen
Source Pooled antisera from goats hyperimmunized with type I collagen
Cross Adsorption Collagen types II, III, IV, V, and VI
Purification Affinity chromatography on type I collagen covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-UNLB
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.4 mg/mL
Volume 0.5 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-3
FLISA
Other referenced applications for relevant formats include -
Immunohistochemistry-Paraffin Sections 4-15
Immunohistochemistry-Frozen Sections 16-24
Immunocytochemistry 14,24-28
Electron Microscopy 29-31
Flow Cytometry 32
Western Blot 3,10,13-15,26-28,33
Immunoprecipitation 34,35
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2753206
  • Frozen MC4R-KO mouse liver section was stained with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01) and anti-F4/80 followed by secondary antibodies and DAPI.

    Image from Itoh M, Kato H, Suganami T, Konuma K, Marumoto Y, Terai S, et al. Hepatic crown-like structure: a unique histological feature in non-alcoholic steatohepatitis in mice and humans. PLoS One. 2013;8(12):e82163. Figure 2(e)
  • Paraffin embedded rat kidney section post uninephrectomy was stained with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01) followed by a secondary antibody and AEC.

    Image from Wang-Rosenke Y, Khadzhynov D, Loof T, Mika A, Kawachi H, Neumayer H, et al. Tyrosine kinases inhibition by Imatinib slows progression in chronic anti-thy1 glomerulosclerosis of the rat. BMC Nephrol. 2013;14:223. Figure 3(b)
  • Human fibroblasts were stained with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01) followed by an AF594 conjugated secondary antibody.

    Image from Fang F, Liu L, Yang Y, Tamaki Z, Wei J, Marangoni RG, et al. The adipokine adiponectin has potent anti-fibrotic effects mediated via adenosine monophosphate-activated protein kinase: novel target for fibrosis therapy. Arthritis Res Ther. 2012;14:R229. Figure 1(c)
  • Purified Human Type I Collagen (SB Cat. No. 1200-01S) was resolved by electrophoresis, transferred to PVDF membrane, probed with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01), and visualized using Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-HRP (SB Cat. No. 6425-05) secondary antibody and chemiluminescent detection.

Frozen MC4R-KO mouse liver section was stained with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01) and anti-F4/80 followed by secondary antibodies and DAPI.

Image from Itoh M, Kato H, Suganami T, Konuma K, Marumoto Y, Terai S, et al. Hepatic crown-like structure: a unique histological feature in non-alcoholic steatohepatitis in mice and humans. PLoS One. 2013;8(12):e82163. Figure 2(e)
 

Paraffin embedded rat kidney section post uninephrectomy was stained with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01) followed by a secondary antibody and AEC.

Image from Wang-Rosenke Y, Khadzhynov D, Loof T, Mika A, Kawachi H, Neumayer H, et al. Tyrosine kinases inhibition by Imatinib slows progression in chronic anti-thy1 glomerulosclerosis of the rat. BMC Nephrol. 2013;14:223. Figure 3(b)
 

Human fibroblasts were stained with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01) followed by an AF594 conjugated secondary antibody.

Image from Fang F, Liu L, Yang Y, Tamaki Z, Wei J, Marangoni RG, et al. The adipokine adiponectin has potent anti-fibrotic effects mediated via adenosine monophosphate-activated protein kinase: novel target for fibrosis therapy. Arthritis Res Ther. 2012;14:R229. Figure 1(c)
 

Purified Human Type I Collagen (SB Cat. No. 1200-01S) was resolved by electrophoresis, transferred to PVDF membrane, probed with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01), and visualized using Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-HRP (SB Cat. No. 6425-05) secondary antibody and chemiluminescent detection.
 
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References
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