Goat Anti-Human Ig-BGAL

Cat. No.
2010-06  
Size
1.0 mL  
Price (USD)
$95.00 
Specificity Reacts with the heavy and light chains of human IgG, IgM, and IgA
Source Pooled antisera from goats hyperimmunized with human IgG, IgM, and IgA
Cross Adsorption None; may react with immunoglobulins from other species
Purification Affinity chromatography on pooled human immunoglobulins covalently linked to agarose
Isotype Goat IgG
Format/Conjugate BGAL (β-galactosidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline containing 0.1% sodium azide
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-8
FLISA
Flow Cytometry 15,16,18
Other referenced applications for relevant formats include -
Immunocytochemistry 9
Western Blot 10-13
Multiplex 14
Stimulation 15-17
Recommended Dilutions Please refer to product specific Technical Bulletin
References
1. Kouzmitcheva GA, Petrenko VA, Smith GP. Identifying diagnostic peptides for Lyme disease through epitope discovery. Clin Diagn Lab Immunol. 2001;8:150-60. (ELISA)
2. Pancook JD, Beuerlein G, Pecht G, Tang Y, Nie Y, Wu H, et al. In vitro affinity maturation of human IgM antibodies reactive with tumor-associated antigens. Hybrid Hybridomics. 2001;20:383-96. (ELISA)
3. Weitkamp J, Kallewaard N, Kusuhara K, Feigelstock D, Feng N, Greenberg HB, et al. Generation of recombinant human monoclonal antibodies to rotavirus from single antigen-specific B cells selected with fluorescent virus-like particles. J Immunol Methods. 2003;275:223-37. (ELISA)
4. Ohdan H, Zhou W, Tanaka Y, Irei T, Fuchimoto Y, Egawa H, et al. Evidence of immune tolerance to blood group antigens in a case of ABO-incompatible pediatric liver transplantation. Am J Transplant. 2007;7:2190-4. (ELISA)
5. Williams JV, Weitkamp J, Blum DL, LaFleur BJ, Crowe JE Jr. The human neonatal B cell response to respiratory syncytial virus uses a biased antibody variable gene repertoire that lacks somatic mutations. Mol Immunol. 2009;47:407-14. (ELISA)
6. Fan Q, Longnecker R. The Ig-like V-type domain of paired Ig-like type 2 receptor alpha is critical for herpes simplex virus type 1-mediated membrane fusion. J Virol. 2010;84:8664-72. (ELISA)
7. Yang G, Holl TM, Liu Y, Li Y, Lu X, Nicely NI, et al. Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies. J Exp Med. 2013;210:241-56. (ELISA)
8. Martí GP, Mohn KG, Cox RJ, Brokstad KA. The influence of tonsillectomy on total serum antibody levels. Scand J Immunol. 2014;80:377-9. (ELISA)
9. Moody MA, Yates NL, Amos JD, Drinker MS, Eudailey JA, Gurley TC, et al. HIV-1 gp120 vaccine induces affinity maturation in both new and persistent antibody clonal lineages. J Virol. 2012;86:7496-507. (ICC)
10. Moritz DR, Gheyselinck J, Klemenz R. Expression analysis of the soluble and membrane-associated forms of the interleukin-1 receptor-related Tl protein in primary mast cells and fibroblasts. Hybridoma. 1998;17:107-16. (WB)
11. Davenport V, Guthrie T, Findlow J, Borrow R, Williams NA, Heyderman RS. Evidence for naturally acquired T cell-mediated mucosal immunity to Neisseria meningitidis. J Immunol. 2003;171:4263-70. (WB)
12. Botelho-Nevers E, Gouriet F, Lepidi H, Couvret A, Amphoux B, Dessi P, et al. Chronic nasal infection caused by Klebsiella rhinoscleromatis or Klebsiella ozaenae: two forgotten infectious diseases. Int J Infect Dis. 2007;11:423-9. (WB)
13. Parcina M, Miranda-Garcia MA, Durlanik S, Ziegler S, Over B, Georg P, et al. Pathogen-triggered activation of plasmacytoid dendritic cells induces IL-10-producing B cells in response to Staphylococcus aureus. J Immunol. 2013;190:1591-602. (WB)
14. Pochechueva T, Chinarev A, Bovin N, Fedier A, Jacob F, Heinzelmann-Schwarz V. PEGylation of microbead surfaces reduces unspecific antibody binding in glycan-based suspension array. J Immunol Methods. 2014;412:42-52. (Multiplex)
15. Fruehling S, Lee SK, Herrold R, Frech B, Laux G, Kremmer E, et al. Identification of latent membrane protein 2A (LMP2A) domains essential for the LMP2A dominant-negative effect on B-lymphocyte surface immunoglobulin signal transduction. J Virol. 1996;70:6216-26. (FC, Stim)
16. Fruehling S, Swart R, Dolwick KM, Kremmer E, Longnecker R. Tyrosine 112 of latent membrane protein 2A is essential for protein tyrosine kinase loading and regulation of Epstein-Barr virus latency. J Virol. 1998;72:7796-806. (FC, Stim)
17. Koethe S, Zander L, Köster S, Annan A, Ebenfelt A, Spencer J, et al. Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation. J Leukoc Biol. 2011;90:5-19. (Stim)
18. Wiehr S, Bühler P, Gierschner D, Wolf P, Rolle A, Kesenheimer C, et al. Pharmacokinetics and PET imaging properties of two recombinant anti-PSMA antibody fragments in comparison to their parental antibody. Prostate. 2014;74:743-55. (FC)