Goat Anti-Human IgG, Monkey ads-BIOT

Cat. No.
2049-08  
Size
1.0 mg  
Price (USD)
$325.00 
Specificity Reacts with the heavy chain of human IgG
Source Pooled antisera from goats hyperimmunized with human IgG
Cross Adsorption Human IgM and IgA; rhesus (Macaca mulatta) and cynomolgus (Macaca fascicularis) IgG; may react with IgG from other species
Purification Affinity chromatography on human IgG covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 2.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA
FLISA
Other referenced applications for relevant formats include -
Immunoprecipitation 1,2
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795697
  • ELISA plate was coated with purified human IgG, IgM, and IgA, cynomolgus IgG, and rhesus IgG. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Monkey ads-BIOT (SB Cat. No. 2049-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

ELISA plate was coated with purified human IgG, IgM, and IgA, cynomolgus IgG, and rhesus IgG. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Monkey ads-BIOT (SB Cat. No. 2049-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 
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References
1. Zhang Q, Spellman DS, Song Y, Choi B, Hatcher NG, Tomazela D, et al. Generic automated method for liquid chromatography-multiple reaction monitoring mass spectrometry based monoclonal antibody quantitation for preclinical pharmacokinetic studies. Anal Chem. 2014;86:8776-84. (IP)
2. Schroeder KM, Beyer TP, Hansen RJ, Han B, Pickard RT, Wroblewski VJ, et al. Proteolytic cleavage of antigen extends the durability of an anti-PCSK9 monoclonal antibody. J Lipid Res. 2015 44075. pii: jlr.M061903. [Epub ahead of print]. (IP)