ELISA plate was coated with purified human IgG, IgM, and IgA and mouse, bovine, and horse serum. Immunoglobulins and sera were detected with Rabbit Anti-Human IgG(H+L), Mouse/Bovine/Horse SP ads-UNLB (SB Cat. No. 6146-01) followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP (SB Cat. No. 4050-05).