Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP

Cat. No.
6300-05  
Size
1.0 mL  
Price (USD)
$105.00 
Specificity Reacts with the heavy and light chains of goat IgG
Source Pooled antisera from swine hyperimmunized with goat IgG
Cross Adsorption Human, rat, and mouse serum proteins (SP); may react with immunoglobulins from other species and the light chains of other goat immunoglobulins
Purification Affinity chromatography on goat IgG covalently linked to agarose
Isotype Swine IgG
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA
FLISA
Other referenced applications for relevant formats include -
Flow Cytometry 1,2
Immunohistochemistry-Frozen Sections 3
Western Blot 4-7
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796284
  • Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
  • ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
  • TK6 cell (control) and Bid knockout cell lysates were resolved by electrophoresis, transferred to PVDF membrane, and probed with anti-Bid followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05) and chemiluminescent detection.

    Image from Miles MA, Hawkins CJ. Executioner caspases and CAD are essential for mutagenesis induced by TRAIL or vincristine. Cell Death Dis. 2017;8:e3062. Figure 5(a)
  • ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab’)2 Anti-Human IgM-UNLB (SB Cat. No. 2022-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
  • Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
  • ELISA plate was coated with purified human IgG, IgM, and IgA, cynomolgus IgG, and rhesus IgG. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Monkey ads-UNLB (SB Cat. No. 2049-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).

Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
 

ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
 

TK6 cell (control) and Bid knockout cell lysates were resolved by electrophoresis, transferred to PVDF membrane, and probed with anti-Bid followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05) and chemiluminescent detection.

Image from Miles MA, Hawkins CJ. Executioner caspases and CAD are essential for mutagenesis induced by TRAIL or vincristine. Cell Death Dis. 2017;8:e3062. Figure 5(a)
 

ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab’)2 Anti-Human IgM-UNLB (SB Cat. No. 2022-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
 

Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
 

ELISA plate was coated with purified human IgG, IgM, and IgA, cynomolgus IgG, and rhesus IgG. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Monkey ads-UNLB (SB Cat. No. 2049-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
 
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