Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-BIOT

Cat. No.
6425-08  
Size
1.0 mg  
Price (USD)
$105.00 
Specificity Reacts with the heavy and light chains of goat IgG
Source Pooled antisera from donkeys hyperimmunized with goat IgG
Cross Adsorption Human, mouse, rat, hamster, rabbit, chicken, horse, and guinea pig serum proteins (SP); may react with immunoglobulins from other species and the light chains of other goat immunoglobulins
Purification Affinity chromatography on goat IgG covalently linked to agarose
Isotype Donkey IgG
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 2.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA
FLISA
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 1
Western Blot 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796347
  • Frozen newborn mouse cartilage section was stained with Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01; right) followed by Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 6425-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
  • ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 6425-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

Frozen newborn mouse cartilage section was stained with Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01; right) followed by Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 6425-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
 

ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 6425-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 
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References
1. SouthernBiotech published data (IHC-FS, WB)