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  • Goat Anti-Human Ig Fc-UNLB

Goat Anti-Human Ig Fc-UNLB

Cat. No.:
2047-01
Purified Goat Anti-Human Ig Fc antibody for use in ELISA assays.
$81.00
Size Price (USD) Quantity
0.5 mg $81.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-UNLB
Specificity Reacts with the Fc region of human IgG, IgM, and IgA
Source Pooled antisera from goats hyperimmunized with human IgG, IgM, and IgA
Cross Adsorption Human IgG Fab; may react with immunoglobulins from other species and the Fc region of other human immunoglobulins
Purification Method Affinity chromatography on pooled human immunoglobulins covalently linked to agarose
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1,2
FLISA

RRID Number AB_2795681

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

References and Citations

  1. 1. Terato K, Do CT, Cutler D, Waritani T, Shionoya H. Preventing intense false positive and negative reactions attributed to the principle of ELISA to re-investigate antibody studies in autoimmune diseases. J Immunol Methods. 2014;407:15-25. (ELISA)
  2. 2. Zhao Q, Ahmed M, Guo H, Cheung IY, Cheung NV. Alteration of electrostatic surface potential enhances affinity and tumor killing properties of anti-ganglioside GD2 monoclonal antibody hu3F8. J Biol Chem. 2015;290:13017-27. (ELISA)

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  1. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
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  2. FLISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-AF488 (SB Cat. No. 6425-30).
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  3. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  4. ELISA plate was coated with serially diluted Human IgG Fc-UNLB (SB Cat. No. 0160-01). Immunoglobulin was detected with Goat Anti-Human IgG Fc-BIOT (SB Cat. No. 2048-08), Goat Anti-Human Ig Fab-BIOT (SB Cat. No. 2085-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT and Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9130-08 and Cat. No. 9140-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  5. ELISA plate was coated with purified human IgG, IgM, and IgA, cynomolgus IgG, and rhesus IgG. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Monkey ads-UNLB (SB Cat. No. 2049-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
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  6. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit F(ab')<sub>2</sub> Anti-Goat IgG(H+L), Human SP ads-HRP (SB Cat. No. 6026-05).
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  8. Human IgG-UNLB (SB Cat. No. 0150-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Mouse Anti-Human IgG Fc-UNLB (SB Cat. No. 9040-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
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  9. FLISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-PE (SB Cat. No. 6157-09).
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  11. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG-UNLB (SB Cat. No. 2040-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  13. Frozen newborn mouse cartilage section was stained with Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01) followed by Mouse Anti-Goat IgG Fc-AF555 (SB Cat. No. 6158-32) and DAPI.
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  14. Human peripheral blood lymphocytes were stained with Goat Anti-Human IgM-BIOT (SB Cat. 2020-01) and Mouse Anti-Human CD19-PE (SB Cat. No. 9340-09) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-FITC (SB Cat. No. 6300-02).
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  15. Human IgG-UNLB (SB Cat. No. 0150-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Mouse Anti-Human IgG Fc-UNLB (SB Cat. No. 9040-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
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  16. ELISA plate was coated with purified human IgG, IgM, and IgA, and mouse IgG, IgM, and IgA (mouse Ig). Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Mouse ads-UNLB (SB Cat. No. 2044-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
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  17. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Kappa-UNLB (SB Cat. No. 0151K-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  19. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG Fc-UNLB (SB Cat. No. 9042-01) followed by Goat Anti-Mouse IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 1038-08) and Streptavidin-HRP (SB Cat. No. 7100-05).
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  20. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type VI Collagen-UNLB (SB Cat. No. 1360-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-BIOT (SB Cat. No. 6300-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
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  21. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Lambda-UNLB (SB Cat. No. 0151L-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  22. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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