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  • Goat Anti-Human Kappa, Monkey ads-BIOT

Goat Anti-Human Kappa, Monkey ads-BIOT

Cat. No.:
2064-08
Goat Anti-Human Kappa-Biotin antibody with minimal reactivity to rhesus and cynomolgus IgG for use in ELISA and flow cytometry assays.
$399.00
Size Price (USD) Quantity
0.5 mg $399.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Specificity Reacts with human κ light chains
Source Pooled antisera from goats hyperimmunized with human κ light chains
Cross Adsorption Human λ light chains; rhesus and cynomolgus IgG; may react with κ light chains from other species
Purification Method Affinity chromatography on human κ light chains covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA
FC

RRID Number AB_2819128
Gene ID 3514 (Human)
Gene ID Symbol IGKC (Human)
Gene ID Aliases HCAK1D; Km; IGKC
UniProt ID P01834 (Human)
UniProt Name IGKC_HUMAN (Human)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat IgG-BIOT (SB Cat No. 0109-08; gray) and Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08) then Streptavidin-FITC (SB Cat. No. 7100-02).
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  2. ELISA plate was coated with purified human IgG, IgM, and IgA, cynomolgus IgG, and rhesus IgG. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Monkey ads-BIOT (SB Cat. No. 2049-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  3. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Kappa-UNLB (SB Cat. No. 0151K-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
    Human IgG1 Kappa-UNLB
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  4. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa-BIOT (SB Cat. No. 2060-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Human Kappa-BIOT
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  5. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  6. Goat F(ab')2 Anti-Human Kappa, Mouse ads-BIOT
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  7. ELISA plate was coated with serially diluted Human IgA Kappa-UNLB (SB Cat. No. 0155K-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
    Human IgA Kappa-UNLB
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  8. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Kappa-BIOT (SB Cat. No. 2062-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat F(ab')2 Anti-Human Kappa-BIOT
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  9. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  10. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda-BIOT (SB Cat. No. 2070-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Human Lambda-BIOT
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  11. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Lambda, Mouse ads-BIOT (SB Cat. No. 2073-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat F(ab')2 Anti-Human Lambda, Mouse ads-BIOT
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  12. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
    Normal Goat Serum
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  13. CD19+ human B-lymphocytes were stained with Goat Anti-Human Lambda, Mouse ads-BIOT (SB Cat. 2071-08) and Goat F(ab')<sub>2</sub> Anti-Human Kappa, Mouse ads-PE (SB Cat. No. 2063-09) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
    Goat Anti-Human Lambda, Mouse ads-BIOT
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    $301.00
  14. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Lambda-BIOT (SB Cat. No. 2072-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat F(ab')2 Anti-Human Lambda-BIOT
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