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  • Goat Anti-Mouse IgG3, Human/Bovine/Horse SP ads-HRP

Goat Anti-Mouse IgG3, Human/Bovine/Horse SP ads-HRP

Cat. No.:
1103-05
Goat Anti-Mouse IgG3-HRP antibody with minimal reactivity to human, bovine, and horse serum proteins for use in ELISA assays.
$134.00
Size Price (USD) Quantity
1.0 mL $134.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control 0109-05
Specificity Reacts with the heavy chain of mouse IgG3
Source Pooled antisera from goats hyperimmunized with mouse IgG3
Cross Adsorption Mouse IgG1, IgG2a, IgG2b, IgM, and IgA; human, bovine, and horse serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG3 covalently linked to agarose
Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality Polyclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA

RRID Number AB_2794595
Gene ID 380795 (Mouse)
Gene ID Symbol Ighg3 (Mouse)
Gene ID Aliases IgG3
UniProt ID P03987 (Mouse)
UniProt Name IGHG3_MOUSE (Mouse)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. ELISA plate was coated with serially diluted Mouse IgG<sub>3</sub>-UNLB (SB Cat. No. 0105-01). Immunoglobulin was detected with Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08), Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-BIOT (SB Cat. No. 1080-08), Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-BIOT (SB Cat. No. 1090-08), Goat Anti-Mouse IgG<sub>3</sub>, Human ads-BIOT (SB Cat. No. 1100-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  2. ELISA plate was coated with purified mouse IgG, IgM, and IgA and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Ig, Human ads-UNLB (SB Cat. No. 1010-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  3. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  4. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>3</sub>-HRP (SB Cat. No. 1101-05).
    Goat Anti-Mouse IgG3-HRP
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  5. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>, Human ads-HRP (SB Cat. No. 1070-05).
    Goat Anti-Mouse IgG1, Human ads-HRP
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  6. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  7. ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM, Human ads-HRP (SB Cat. No. 1020-05).
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  8. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-HRP (SB Cat. No. 1080-05).
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  9. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-HRP (SB Cat. No. 1090-05).
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  10. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>3</sub>, Human ads-HRP (SB Cat. No. 1100-05).
    Goat Anti-Mouse IgG3, Human ads-HRP
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  11. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgG<sub>3</sub>-HRP (SB Cat. No. 1191-05).
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  12. ELISA plate was coated with purified mouse IgA, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgA-HRP (SB Cat. No. 1040-05).
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  13. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>2c</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2c</sub>, Human ads-HRP (SB Cat. No. 1079-05).
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  14. Lane 1 - Mouse IgG<sub>1</sub>κ<br/>Lane 2 - Mouse IgG<sub>2a</sub>λ<br/>Lane 3 - Mouse IgG<sub>2b</sub>κ<br/>Lane 4 - Mouse IgG<br/>Mouse immunoglobulins above were resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Goat Anti-Mouse Kappa-HRP (SB Cat. No. 1050-05; 1:4K). Proteins were visualized using chemiluminescent detection.
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  15. Lane 1 - Mouse IgG<sub>1</sub>κ<br/>Lane 2 - Mouse IgG<sub>2a</sub>λ<br/>Lane 3 - Mouse IgG<sub>2b</sub>κ<br/>Mouse immunoglobulins above were resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Goat Anti-Mouse Lambda-HRP (SB Cat. No. 1060-05; 1:4K). Proteins were visualized using chemiluminescent detection.
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  16. ELISA plate was coated with purified mouse IgE, IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgE-HRP (SB Cat. No. 1110-05).
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