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Hamster Anti-Mouse CD3ε-LE/AF (500-A2)

Cat. No.:
Low Endotoxin/Azide Free Anti-Mouse CD3 epsilon antibody for use in flow cytometry, immunohistochemistry, immunoprecipitation, and activation assays.
Size Price (USD) Quantity
0.5 mg $218.00
More Information
Clone 500-A2
Isotype Hamster (Syrian) IgG2
Isotype Control Hamster IgG-LE/AF
Specificity Mouse CD3ε
Alternative Names CD3 epsilon
Description CD3ε, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3ε is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. 500-A2 recognizes an epitope on the 25 kDa ε chain of the CD3/TCR complex of mouse strains tested.
Immunogen Immune complexes isolated from C6VL-BS thymoma cells
Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Handle under aseptic conditions
Applications Flow Cytometry – Quality tested 1
Immunohistochemistry-Frozen Sections – Reported in literature 3
Immunoprecipitation – Reported in literature 1
Activation – Reported in literature 1,2,4

RRID Number AB_2794815
Gene ID 12501 (Mouse)
Gene ID Symbol Cd3e (Mouse)
Gene ID Aliases AI504783; CD3; CD3epsilon; T3e
UniProt ID P22646 (Mouse
UniProt Name CD3E_MOUSE (Mouse)

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  1. 1. Allison JP, Havran WL, Poenie M, Kimura J, DeGraffenreid L, Ajami M, et al. Expression and function of CD3 on murine thymocytes. In: Davis MM, Kappler J, editors. The T-Cell Receptor, UCLA Symposia, 73rd Edition. New York: Alan R. Liss; 1988. p.33-45. (Immunogen, FC, IP, Activ)
  2. 2. Swallow MM, Wallin JJ, Sha WC. B7h, a novel costimulatory homolog of B7.1 and B7.2, is induced by TNFα. Immunity. 1999;11:423-32. (Activ)
  3. 3. Hörnquist CE, Lu X, Rogers-Fani PM, Rudolph U, Shappell S, Birnbaumer L, et al. Gαi2-deficient mice with colitis exhibit a local increase in memory CD4+ T cells and proinflammatory Th1-type cytokines. J Immunol. 1997;158:1068-77. (IHC-FS)
  4. 4. Portoles P, Rojo J, Golby A, Bonneville M, Gromkowski S, Greenbaum L, et al. Monoclonal antibodies to murine CD3ε define distinct epitopes one of which may interact with CD4 during T cell activation. J Immunol. 1989;142:4169-75. (Activ)
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