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Mouse Anti-Chicken Bu-1a-FITC (21-1A4)

Cat. No.:
FITC Anti-Chicken Bu-1a antibody for use in flow cytometry and immunohistochemistry / immunocytochemistry assays.
Size Price (USD) Quantity
0.5 mg $374.00
More Information
Clone 21-1A4
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-FITC (15H6)
Specificity Chicken Bu-1a
Alternative Names ChB6.1
Description Chicken Bu-1a, a product of the Bu-1a allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes but not on granulocytes, erythrocytes, or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion. The monoclonal antibody 21-1A4 does not react with cells from CHA and H.B14ab strains by immunofluorescence.
Immunogen Bursa cells from one-day-old H.B15 (Bu-1a/b) strain chickens
Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Applications Flow Cytometry – Quality tested 1,5-9
Immunohistochemistry-Frozen Sections – Reported in literature 2
Immunocytochemistry – Reported in literature 1
Immunoprecipitation – Reported in literature 3,4
Apoptosis – Reported in literature 4

RRID Number AB_2796522
Gene ID 396098 (Chicken)
Gene ID Symbol LOC396098 (Chicken)
Gene ID Aliases B6.2; Bu-1; Bu-1b

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  1. 1. Veromaa T, Vainio O, Eerola E, Toivanen P. Monoclonal antibodies against chicken Bu-1a and Bu-1b alloantigens. Hybridoma. 1988;7:41-8. (Immunogen, ICC, FC)
  2. 2. Sayegh CE, Ratcliffe MJ. Perinatal deletion of B cells expressing surface Ig molecules that lack V(D)J-encoded determinants in the bursa of Fabricius is not due to intrafollicular competition. J Immunol. 2000;164:5041-8. (IHC-FS)
  3. 3. Huffnagle GB, Ratcliffe MJ, Humphries EH. Bu-2, a novel avian cell surface antigen on B cells and a population of non-lymphoid cells, is expressed homogeneously in germinal centers. Hybridoma. 1989;8:589-604. (FC, IP)
  4. 4. Funk PE, Pifer J, Kharas M, Crisafi G, Johnson A. The avian chB6 alloantigen induces apoptosis in DT40 B cells. Cell Immunol. 2003;226:95-104. (IP, Apop)
  5. 5. Ratcliffe MJ. Generation of immunoglobulin heavy chain diversity subsequent to cell surface immunoglobulin expression in the avian bursa of Fabricius. J Exp Med. 1989;170:1165-73. (FC)
  6. 6. Pararnithiotis E, Jacobsen KA, Ratcliffe MJ. Loss of surface immunoglobulin expression precedes B cell death by apoptosis in the bursa of Fabricius. J Exp Med. 1995;181:105-13. (FC)
  7. 7. Sayegh CE, Demaries SL, Iacampo S, Ratcliffe MJ. Development of B cells expressing surface immunoglobulin molecules that lack V(D)J-encoded determinants in the avian embryo bursa of fabricius. Proc Natl Acad Sci USA. 1999;96:10806-11. (FC)
  8. 8. Hunt HD, Goto RM, Foster DN, Bacon LD, Miller MM. At least one YMHCI molecule in the chicken is alloimmunogenic and dynamically expressed on spleen cells during development. Immunogenetics. 2006;58:297-307. (FC)
  9. 9. Alitheen NB, McClure SJ, Yeap SK, Kristeen-Teo YW, Tan SW, McCullagh P. Establishment of an in vitro system representing the chicken gut-associated lymphoid tissue. PLoS One. 2012;7(11):e49188. (FC)
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