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Mouse Anti-Human CD11b-AF488 (ICRF44)
Cat. No.:
9546-30
Alexa Fluor® 488 Anti-Human CD11b antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blot assays.
$266.00
| Clone | ICRF44 |
|---|---|
| Isotype | Mouse (BALB/c) IgG1κ |
| Isotype Control | Mouse IgG1-AF488 (15H6) |
| Specificity | Human/Porcine/Rhesus/Cynomolgus/Chimpanzee/Baboon CD11b |
| Alternative Names | Mac-1α, Mo-1, CR3, integrin αM, C3biR, ITGAM, CR3A, p170 |
| Description | CD11b is the 165 kDa αM chain which combines with the CD18 β2 integrin subunit to form the Mac-1 heterodimer. It is expressed on activated lymphocytes, monocytes, granulocytes and a subset of NK cells. CD11b functions in cell-cell and cell-substrate interactions and is a ligand for C3bi, CD50 (ICAM-3), CD54 (ICAM-1) and CD102 (ICAM-2). |
| Immunogen | Rheumatoid synovial cells and human monocytes |
| Conjugate | AF488 (Alexa Fluor® 488) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications |
Flow Cytometry – Quality tested 7,9 Immunohistochemistry-Frozen Sections – Reported in literature 3 Immunocytochemistry – Reported in literature 4 Immunoprecipitation – Reported in literature 1 Western Blot – Reported in literature 2 Blocking – Reported in literature 5,6 |
| RRID Number | AB_2796909 |
| Gene ID |
3684 (Human) 397459 (Porcine) 714832 (Rhesus) 101866035 (Cynomolgus) 454069 (Chimpanzee) 101004087 (Baboon) |
| Gene ID Symbol |
ITGAM (Human) ITGAM (Porcine) ITGAM (Rhesus) ITGAM (Cynomolgus) ITGAM (Chimpanzee) ITGAM (Baboon) |
| Gene ID Aliases | CD11B; CR3A; MAC-1; MAC1A; MO1A; SLEB6; CK820_G0050589 |
| UniProt ID |
P11215 (Human) |
| UniProt Name |
ITAM_HUMAN (Human) |
Documentation
Certificate of Analysis Lookup
Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view
- 1. Feng C, Zhang L, Almulki L, Faez S, Whitford M, Hafezi-Moghadam A, et al. Endogenous PMN sialidase activity exposes activation epitope on CD11b/CD18 which enhances its binding interaction with ICAM-1. J Leukoc Biol. 2011;90:313-21. (IP)
- 2. David A, Kacher Y, Specks U, Aviram I. Interaction of proteinase 3 with CD11b/CD18 (β2 integrin) on the cell membrane of human neutrophils. J Leukoc Biol. 2003;74:551-7. (WB)
- 3. Mayer WJ, Klaproth OK, Hengerer FH, Kook D, Dirisamer M, Priglinger S, et al. In vitro immunohistochemical and morphological observations of penetrating corneal incisions created by a femtosecond laser used for assisted intraocular lens surgery. J Cataract Refract Surg. 2014;40:632-8. (IHC-FS)
- 4. Friedenberger M, Bode M, Krusche A, Schubert W. Fluorescence detection of protein clusters in individual cells and tissue sections by using toponome imaging system: sample preparation and measuring procedures. Nat Protoc. 2007;2:2285-94. (ICC)
- 5. Malhotra V, Hogg N, Sim RB. Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3). Eur J Immunol. 1986;16:1117-23. (Block)
- 6. Nasr AB, Haithcoat J, Masterson JE, Gunn JS, Eaves-Pyles T, Klimpel GR. Critical role for serum opsonins and complement receptors CR3 (CD11b/CD18) and CR4 (CD11c/CD18) in phagocytosis of Francisella tularensis by human dendritic cells (DC): uptake of Francisella leads to activation of immature DC and intracellular survival of the bacteria. J Leukoc Biol. 2006;80:774-86. (Block)
- 7. Lester EA, Babensee JE. Proinflammatory phenotype of endothelial cells after coculture with biomaterial-treated blood cells. J Biomed Mater Res A. 2003;64:397-410. (FC)
- 8. Hérodin F, Thullier P, Garin M, Drouet M. Nonhuman primates are relevant models for research in hematology, immunology and virology. Eur Cytokine Netw. 2005;16:104-16. (Chimpanzee, Baboon, Cynomolgus, Rhesus Reactivity)
- 9. Singh S, Kloss FR, Brunauer R, Schimke M, Jamnig A, Greiderer-Kleinlercher B, et al. Mesenchymal stem cells show radioresistance in vivo. J Cell Mol Med. 2012;16:877-87. (FC, Porcine Reactivity)
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