Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

Mouse Anti-Human CD33-AF700 (WM53)

Cat. No.:
Alexa Fluor® 700 Anti-Human CD33 antibody for use in flow cytometry assays.
Size Price (USD) Quantity
100 tests $259.00
More Information
Clone WM53
Isotype Mouse IgG1κ
Isotype Control Mouse IgG1-AF700 (15H6)
Specificity Human CD33
Alternative Names gp67, p67, Siglec-3
Description CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function.
Conjugate AF700 (Alexa Fluor® 700)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Flow Cytometry – Quality tested 1,2,4-6
Immunohistochemistry-Frozen Sections – Reported in literature 3
Immunocytochemistry – Reported in literature 4
Immunoprecipitation – Reported in literature 1
Western Blot – Reported in literature 2
Blocking – Reported in literature 1,5,6

RRID Number AB_2796992
Gene ID 945 (Human)
Gene ID Symbol CD33 (Human)
Gene ID Aliases SIGLEC-3; SIGLEC3; p67
UniProt ID P20138 (Human
UniProt Name CD33_HUMAN (Human)

Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view

Need help with this product?
or call 800.722.2255
  1. 1. Hernández-Caselles T, Martínez-Esparza M, Pérez-Oliva AB, Quintanilla-Cecconi AM, García-Alonso A, Alvarez-Lopéz DM, et al. A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing. J Leukoc Biol. 2006;79:46-58. (IP, FC, Block)
  2. 2. Garnache-Ottou F, Chaperot L, Biichle S, Ferrand C, Remy-Martin J, Deconinck E, et al. Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells. Blood. 2005;105:1256-64. (WB, FC)
  3. 3. Lampinen M, Waddell A, Ahrens R, Carlson M, Hogan SP. CD14+CD33+ myeloid cell-CCL11-eosinophil signature in ulcerative colitis. J Leukoc Biol. 2013;94:1061-70. (IHC-FS)
  4. 4. Pérez-Oliva AB, Martínez-Esparza M, Vicente-Fernández JJ, Corral-San Miguel R, Garcia-Peñarrubia P, Hernández-Caselles T. Epitope mapping, expression and post-translational modifications of two isoforms of CD33 (CD33M and CD33m) on lymphoid and myeloid human cells. Glycobiology. 2011;21:757-70. (ICC, FC)
  5. 5. Vitale C, Romagnani C, Falco M, Ponte M, Vitale M, Moretta A, et al. Engagement of p75/AIRM1 or CD33 inhibits the proliferation of normal or leukemic myeloid cells. Proc Natl Acad Sci USA. 1999;96:15091-6. (FC, Block)
  6. 6. Vitale C, Romagnani C, Puccetti A, Olive D, Costello R, Chiossone L, et al. Surface expression and function of p75/AIRM-1 or CD33 in acute myeloid leukemias: engagement of CD33 induces apoptosis of leukemic cells. Proc Natl Acad Sci USA. 2001;98:5764-9. (FC, Block)
See All References