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Mouse Anti-Human CD8-PE (UCHT4)

Cat. No.:
PE Anti-Human CD8 antibody for use in flow cytometry assays.
Size Price (USD) Quantity
100 tests $218.00
More Information
Clone UCHT4
Isotype Mouse (BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-PE (HOPC-1)
Specificity Human/Rhesus/Cynomolgus/Chimpanzee CD8
Alternative Names Leu-2, Lyt2, Tp32, T8
Description CD8 is a 33 kDa transmembrane glycoprotein expressed as either a CD8αα homodimer or CD8αβ heterodimer. It is expressed on the “cytotoxic/suppressor” subpopulation of peripheral T cells. CD8 functions primarily as a coreceptor with MHC class I-restricted TCR’s in antigen recognition.
Immunogen Thymocytes and Sézary T cells
Conjugate PE (R-phycoerythrin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer
Clonality Monoclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light; Do not freeze
Applications Flow Cytometry – Quality tested 1,2
Immunohistochemistry-Frozen Sections – Reported in literature 3-5
Immunohistochemistry-Paraffin Sections – Reported in literature 6
Immunoprecipitation – Reported in literature 1
Separation – Reported in literature 2
Blocking – Reported in literature 1,7

RRID Number AB_2796875
Gene ID 925 (Human)
698329 (Rhesus)
102124337 (Cynomolgus)
735755 (Chimpanzee)
Gene ID Symbol CD8A (Human)
CD8A (Rhesus)
CD8A (Cynomolgus)
CD8A (Chimpanzee)
Gene ID Aliases CD8; p32; Leu2; CK820_G0032528
UniProt ID P01732 (Human
UniProt Name CD8A_HUMAN (Human)

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  1. 1. Martin PJ, Ledbetter JA, Clark EA, Beatty PG, Hansen JA. Epitope mapping of the human surface suppressor/cytotoxic T cell molecule Tp32. J Immunol. 1984;132:759-65. (FC, IP, Block)
  2. 2. Satyaraj E, Rath S, Bal V. Induction of tolerance in freshly isolated alloreactive T cells by activated T cell stimulators is not due to the absence of CD28-B7 interaction. J Immunol. 1995;155:4669-75. (FC, Sep)
  3. 3. Dogan A, Dunn-Walters DK, MacDonald TT, Spencer J. Demonstration of local clonality of mucosal T cells in human colon using DNA obtained by microdissection of immunohistochemically stained tissue sections. Eur J Immunol. 1996;26:1240-5. (IHC-FS)
  4. 4. Kap YS, van Meurs M, van Driel N, Koopman G, Melief M, Brok HP, et al. A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates. J Histochem Cytochem. 2009;57:1159-67. (IHC-FS, Chimpanzee, Rhesus, Cynomolgus Reactivity)
  5. 5. Loginov R, Halme L, Arola J, Höckerstedt K, Lautenschlager I. Intragraft immunological events associated with EBV DNAemia in liver transplant patients. APMIS. 2010;118:888-94. (IHC-FS)
  6. 6. Martín AP, Ortiz S, Cabalier ME, Frede S, Burgos E, Hliba E, et al. Vascular endothelium express CS-1 fibronectin in allergic contact dermatitis. J Cutan Pathol. 2002;29:347-53. (IHC-PS)
  7. 7. Olsson T, Bakhiet M, Höjeberg B, Ljungdahl Å, Edlund C, Andersson G, et al. CD8 is critically involved in lymphocyte activation by a T. brucei brucei-released molecule. Cell. 1993;72:715-27. (Block)
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