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  • Mouse Anti-Human IgG Fc-BIOT (JDC-10-R - Recombinant)

Mouse Anti-Human IgG Fc-BIOT (JDC-10-R - Recombinant)

Cat. No.:
29040-08
Biotin Anti-Human IgG Fc recombinant antibody for use in ELISA and flow cytometry assays.
$110.00
Size Price (USD) Quantity
0.25 mg $110.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone JDC-10-R
(Genetically derived from DNA sequences of a parental hybridoma clone; for details on the parental clone, see Cat. No. 9040)
Isotype Mouse IgG1κ
Isotype Control Mouse IgG1-BIOT (15H6)
Specificity Human IgG Fc
Immunogen Purified human IgG1 myeloma protein
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.5 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested
Flow Cytometry – Quality tested

RRID Number AB_3076630

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. ELISA plate was coated with Goat Anti-Mouse IgG<sub>1</sub>, Human ads-UNLB (SB Cat. No. 1070-01), Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-UNLB (SB Cat. No. 1080-01), Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-UNLB (SB Cat. No. 1090-01), Goat Anti-Mouse IgG<sub>3</sub>, Human ads-UNLB (SB Cat. No. 1100-01), Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01), and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01). Serially diluted Mouse IgG<sub>1</sub>-BIOT (SB Cat. No. 0102-08) was captured followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
    Mouse IgG1-BIOT (15H6)
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  2. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG Fc-BIOT (SB Cat. No. 9040-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Human IgG Fc-BIOT (JDC-10)
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  3. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG Fc-BIOT (SB Cat. No. 9042-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Human IgG Fc-BIOT (H2)
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  4. ELISA plate was coated with purified human IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG Fc-BIOT (SB Cat. No. 2048-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Human IgG Fc-BIOT
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  5. Human IgG-UNLB (SB Cat. No. 0150-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Mouse Anti-Human IgG Fc-UNLB (SB Cat. No. 9040-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
    Human IgG-UNLB
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  6. ELISA plate was coated with purified human IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG Fc, Multi-Species SP ads-BIOT (SB Cat. No. 2014-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  7. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
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  8. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  9. FLISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>2b</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Mouse Anti-Biotin-FITC (SB Cat. No. 6404-02).
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  10. ELISA plate was coated with serially diluted Human IgG Fc-UNLB (SB Cat. No. 0160-01). Immunoglobulin was detected with Goat Anti-Human IgG Fc-BIOT (SB Cat. No. 2048-08), Goat Anti-Human Ig Fab-BIOT (SB Cat. No. 2085-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT and Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9130-08 and Cat. No. 9140-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Human IgG Fc-UNLB
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