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  • Mouse Anti-Human IgG2 Fc-UNLB (31-7-4 -R- Recombinant)

Mouse Anti-Human IgG2 Fc-UNLB (31-7-4 -R- Recombinant)

Cat. No.:
29060-01
Purified Anti-Human IgG2 Fc recombinant antibody for use in ELISA assays.
$85.00
Size Price (USD) Quantity
0.25 mg $85.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone 31-7-4-R
(Genetically derived from DNA sequences of a parental hybridoma clone; for details on the parental clone, see Cat. No. 9060)
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-UNLB (15H6)
Specificity Human IgG2 Fc
Immunogen Human IgG2 myeloma protein
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.5 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested

Gene ID 3501 (Human)
Gene ID Symbol IGHG2 (Human)
UniProt ID P01859 (Human)
UniProt Name IGHG2_HUMAN (Human)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

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  1. Frozen newborn mouse cartilage section was stained with Mouse IgG<sub>1</sub>-UNLB isotype control (SB Cat. No. 0102-01; left) and Mouse Anti-Type II Collagen-UNLB (right) followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-FITC (SB Cat. No. 1070-02) and DAPI.
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  2. ELISA plate was coated with purified human IgG<sub>1</sub>, IgG<sub>2</sub>, IgG<sub>3</sub>, and IgG<sub>4</sub>. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG<sub>1</sub> Hinge-UNLB (SB Cat. No. 9052-01) followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-HRP (SB Cat. No. 1070-05).
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  3. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  4. Paraffin embedded tubular basement membrane tissue section from patients with Anti-GBM disease was stained with Mouse Anti-Human IgG<sub>3</sub> Hinge-UNLB (SB Cat. No. 9210-01) followed by an HRP conjugated secondary antibody and DAB.<br/>Image from Qu Z, Cui Z, Liu G, Zhao M. The distribution of IgG subclass deposition on renal tissues from patients with anti-glomerular basement membrane disease. BMC Immunol. 2013;14:19. Figure 1(f)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/2.0/
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  5. ELISA plate was coated with purified human IgG<sub>1</sub>, IgG<sub>2</sub>, IgG<sub>3</sub>, and IgG<sub>4</sub>. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG<sub>4</sub> Fc-UNLB (SB Cat. No. 9200-01) followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-HRP (SB Cat. No. 1070-05).
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  6. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>, Human/Bovine/Horse SP ads-HRP (SB Cat. No. 1073-05).
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  7. Human IgG-UNLB (SB Cat. No. 0150-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Mouse Anti-Human IgG Fc-UNLB (SB Cat. No. 9040-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
    Human IgG-UNLB
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  8. Paraffin embedded glomerular basement membrane tissue sections from patients with Anti-GBM disease were stained with Mouse Anti-Human IgG<sub>2</sub> Fd-UNLB (SB Cat. No. 9080-01; right) followed by an HRP conjugated secondary antibody and DAB.<br/>Images from Qu Z, Cui Z, Liu G, Zhao M. The distribution of IgG subclass deposition on renal tissues from patients with anti-glomerular basement membrane disease. BMC Immunol. 2013;14:19. Figures 1(b,e)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/2.0/
    Mouse Anti-Human IgG2 Fd-UNLB (HP6014)
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  9. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgG<sub>1</sub>-AF647 (SB Cat. No. 1144-31).
    Rat Anti-Mouse IgG1-AF647 (SB77e)
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