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  • Mouse Anti-Human Lambda-UNLB (JDC-12-R - Recombinant)

Mouse Anti-Human Lambda-UNLB (JDC-12-R - Recombinant)

Cat. No.:
29180-01
Purified Anti-Human Lambda recombinant antibody for use in ELISA and flow cytometry assays.
$80.00
Size Price (USD) Quantity
0.25 mg $80.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone JDC-12-R
(Genetically derived from DNA sequences of a parental hybridoma clone; for details on the parental clone, see Cat. No. 9180)
Isotype Mouse IgG1κ
Isotype Control Mouse IgG1-UNLB (15H6)
Specificity Human Lambda
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.5 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested
Flow Cytometry – Quality tested

RRID Number AB_3076633
Gene ID 3535 (Human)
Gene ID Symbol IGL (Human)
Gene ID Aliases IGL@; IGLC6

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. Frozen newborn mouse cartilage section was stained with Mouse IgG<sub>1</sub>-UNLB isotype control (SB Cat. No. 0102-01; left) and Mouse Anti-Type II Collagen-UNLB (right) followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-FITC (SB Cat. No. 1070-02) and DAPI.
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  2. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Mouse Anti-Human Lambda-UNLB (SB Cat. No. 9180-01) followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-HRP (SB Cat. No. 1070-05).
    Mouse Anti-Human Lambda-UNLB (JDC-12)
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  3. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda-UNLB (SB Cat. No. 2070-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
    Goat Anti-Human Lambda-UNLB
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  4. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Lambda-UNLB (SB Cat. No. 0151L-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
    Human IgG1 Lambda-UNLB
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  5. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  6. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Mouse Anti-Human Kappa-UNLB (SB Cat. No. 9230-01) followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-HRP (SB Cat. No. 1070-05).
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  7. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa-UNLB (SB Cat. No. 2060-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  8. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>, Human ads-AF647 (SB Cat. No. 1070-31).
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  9. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Kappa-UNLB (SB Cat. No. 0151K-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  10. Goat Anti-Human Lambda, Mouse ads-UNLB
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  11. CD19+ human B-lymphocytes were stained with Goat Anti-Human Kappa, Mouse ads-UNLB (SB Cat. 2061-01) and Goat F(ab')<sub>2</sub> Anti-Human Lambda, Mouse ads-PE (SB Cat. No. 2073-09) followed by Mouse Anti-Goat IgG Fc-AF488 (SB Cat. No. 6158-30).
    Goat Anti-Human Kappa, Mouse ads-UNLB
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  12. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgG<sub>1</sub>-PE (SB Cat. No. 1144-09).
    Rat Anti-Mouse IgG1-PE (SB77e)
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  13. ELISA plate was coated with serially diluted Human IgA Lambda-UNLB (SB Cat. No. 0155L-01). Immunoglobulin was detected with Mouse Anti-Human IgG-BIOT (SB Cat. No. 9040-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  15. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Kappa-UNLB (SB Cat. No. 2062-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/ Mouse SP ads-HRP (SB Cat. No. 6300-05).
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  16. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>, Human/Bovine/Horse SP ads-HRP (SB Cat. No. 1073-05).
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