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Mouse Anti-Mouse I-Ab-BIOT (25-9-3)

Cat. No.:
Biotin Anti-Mouse I-Ab antibody for use in flow cytometry and immunohistochemistry assays.
Size Price (USD) Quantity
0.5 mg $248.00
More Information
Clone 25-9-3
Isotype Mouse (C3H) IgMκ
Isotype Control Mouse IgM-BIOT (11E10)
Specificity Mouse I-Ab
Alternative Names MHC Class II
Description The monoclonal antibody 25-9-3 reacts specifically with the I-Ab haplotype of MHC Class II molecules. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells, and certain epithelial cells.
Immunogen C3H.SW mouse splenocytes
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Flow Cytometry – Quality tested
Immunohistochemistry-Frozen Sections – Reported in literature 2
Complement Mediated Cell Depletion – Reported in literature 3-6

RRID Number AB_2795450
Gene ID 14961 (Mouse)
Gene ID Symbol H2-Ab1 (Mouse)
Gene ID Aliases AI845868; Abeta; H-2Ab; H2-Ab; I-Abeta; IAb; Ia-2; Ia2; Rmcs1
UniProt ID P14483 (Mouse)
UniProt Name HB2A_MOUSE (Mouse)

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  1. 1. Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J Immunol. 1981;126:317-21. (Immunogen)
  2. 2. Komano H, Fujiura Y, Kawaguchi M, Matsumoto S, Hashimoto Y, Obana S, et al. Homeostatic regulation of intestinal epithelia by intraepithelial γδ T cells. Proc Natl Acad Sci USA. 1995;92:6147-51. (IHC-FS)
  3. 3. Sperling AI, Green JM, Mosley RL, Smith PL, DiPaolo RJ, Klein JR, et al. CD43 is a murine T cell costimulatory receptor that functions independently of CD28. J Exp Med. 1995;182:139-46. (CMDC)
  4. 4. Lenschow DJ, Sperling AI, Cooke MP, Freeman G, Rhee L, Decker DC, et al. Differential up-regulation of the B7-1 and B7-2 costimulatory molecules after Ig receptor engagement by antigen. J Immunol. 1994;153:1990-7. (CMDC)
  5. 5. Khattri R, Auger JA, Griffin MD, Sharpe AH, Bluestone JA. Lymphoproliferative disorder in CTLA-4 knockout mice is characterized by CD28-regulated activation of Th2 responses. J Immunol. 1999;162:5784-91. (CMDC)
  6. 6. Rulifson IC, Szot GL, Palmer E, Bluestone JA. Inability to induce tolerance through direct antigen presentation. Am J Transplant. 2002;2:510-9. (CMDC)
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