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Mouse Anti-Mouse I-Ad-UNLB (34-5-3)

Cat. No.:
1901-01
Purified Anti-Mouse I-Ad antibody for use in flow cytometry, immunoprecipitation, activation, blocking, and cytotoxicity assays.
$212.00
Size Price (USD) Quantity
0.5 mg $212.00
More Information
Clone 34-5-3
Isotype Mouse (C3H) IgG2aκ
Isotype Control Mouse IgG2a-UNLB (HOPC-1)
Specificity Mouse I-Ad
Alternative Names MHC Class II, I-bd, I-Aβ, H2-I/Adβ
Description The monoclonal antibody 34-5-3 reacts with the β chain of the I-Ad MHC class II alloantigen. It cross-reacts with I-Ab and with cells from mice of the H-2p and H-2q halotypes.
Immunogen (C57BL/6 x DBA/2)F1 mouse splenocytes
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Flow Cytometry – Quality tested 5
Immunoprecipitation – Reported in literature 2
Activation – Reported in literature 4
Blocking – Reported in literature 3
Cytoxicity – Reported in literature 1

RRID Number AB_2795478
Gene ID 14961 (Mouse)
Gene ID Symbol H2-Ab1 (Mouse)
Gene ID Aliases IAb; Ia2; Ia-2; Abeta; H-2Ab; H2-Ab; Rmcs1; I-Abeta; AI845868
UniProt ID P01921 (Mouse)
UniProt Name HB2D_MOUSE (Mouse)

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  1. 1. Ozato K, Mayer NM, Sachs DH. Monoclonal antibodies to mouse major histocompatibility complex antigens. IV. A series of hybridoma clones producing anti-H-2d antibodies and an examination of expression of H-2d antigens on the surface of these cells. Transplantation. 1982;34:113-9. (Immunogen, Cyto)
  2. 2. Bobé P, Benihoud K, Kiger N. Allogenic MHC class II determinant(s) in MRL-Ipr autoimmune disease-prone mice. Unusual expression of an L1 transposable element creates molecular mimicry. J Immunol. 1993;151:2813-9. (IP)
  3. 3. Mozes E, Dayan M, Zisman E, Brocke S, Licht A, Pecht I. Direct binding of a myasthenia gravis related epitope to MHC class II molecules on living murine antigen-presenting cells. EMBO J. 1989;8:4049-52. (Block)
  4. 4. Bishop GA, Frelinger JA. Haplotype-specific differences in signaling by transfected class II molecules to a Ly-1+ B-cell clone. Proc Natl Acad Sci USA. 1989;86:5933-7. (Activ)
  5. 5. Grusby MJ, Johnson RS, Papaioannou VE, Glimcher LH. Deletion of CD4+ T cells in major histocompatibility complex class II-deficient mice. Science. 1991;253:1417-20. (FC)
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