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Mouse Anti-Mouse H-2Db-AF488 (27-11-13S)

Cat. No.:
1913-30
Alexa Fluor® 488 Anti-Mouse H-2Db antibody for use in flow cytometry and immunocytochemistry assays.
$199.00
Size Price (USD) Quantity
0.1 mg $199.00
More Information
Clone 27-11-13S
Isotype Mouse (C3H) IgG2aκ
Isotype Control Mouse IgG2a-AF488 (HOPC-1)
Specificity Mouse H-2Db
Alternative Names MHC Class I
Description The monoclonal antibody 27-11-13S recognizes with the α3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the α3 domain of H-2Dd, H-2Dq, H-2Ld and H-2Lq but not H-2Kb. Reactivity with haplotypes k, f, p, r, and s has not been observed.
Immunogen BDF1 mouse splenocytes
Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.2 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Flow Cytometry – Quality tested 2
Immunocytochemistry – Reported in literature 3
Immunoprecipitation – Reported in literature 4
Complement Mediated Cell Depletion – Reported in literature 1

RRID Number AB_2795509
Gene ID 14964 (Mouse)
Gene ID Symbol H2-D1 (Mouse)
Gene ID Aliases H-2D; H2-D; H2-K1
UniProt ID P01899 (Mouse)
UniProt Name HA11_MOUSE (Mouse)

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  1. 1. Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J Immunol. 1981;126:317-21. (Immunogen, CMDC)
  2. 2. Palmowski MJ, Parker M, Choudhuri K, Chiu C, Callan MF, van der Merwe PA. A single-chain H-2Db molecule presenting an influenza virus nucleoprotein epitope shows enhanced ability at stimulating CD8+ T cell responses in vivo. J Immuno. 2009;182:4565-71. (FC)
  3. 3. Choudhuri K, Parker M, Milicic A, Cole DK, Shaw MK, Sewell AK, et al. Peptide-major histocompatibility complex dimensions control proximal kinase-phosphatase balance during T cell activation. J Biol Chem. 2009;284:26096-105. (ICC)
  4. 4. Shemesh J, Ehrlich R. Aberrant biosynthesis and transport of class I major histocompatibility complex molecules in cells transformed with highly oncogenic human adenoviruses. J Biol Chem. 1993;268:15704-11. (IP)