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Rat Anti-Human IL-10-UNLB (JES3-12G8)

Cat. No.:
10110-01
Purified Anti-Human IL-10 antibody for use in immunohistochemistry, ELISA, ELISpot, and western blot assays.
$200.00
Size Price (USD) Quantity
0.5 mg $200.00
More Information
Clone JES3-12G8
Isotype Rat IgG2aκ
Isotype Control Rat IgG2a-UNLB (KLH/G2a-1-1)
Specificity Human/Viral/Cynomolgus IL-10
Alternative Names Interleukin-10, cytokine synthesis inhibitory factor, CSIF, TGIF, B-TCGF
Immunogen COS-expressed human IL-10
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications ELISA-Detection – Quality tested 1-4,6
ELISpot-Detection – Reported in literature 5-7
Immunohistochemistry-Frozen Sections – Reported in literature 8-10
Immunohistochemistry-Paraffin Sections – Reported in literature 11
Western Blot – Reported in literature 4,12
Neutralization – Reported in literature 1,12,13
Multiplex-Detection – Reported in literature 14

Note – May be paired with the purified clone JES3-9D7 (SB Cat. No. 10100-01) in a sandwich ELISA

RRID Number AB_2794133
Gene ID 3586 (Human)
102133450 (Cynomolgus)
Gene ID Symbol IL10 (Human)
IL10 (Cynomolgus)
Gene ID Aliases CSIF; GVHDS; IL-10; IL10A; TGIF
UniProt ID P22301 (Human)
P79338 (Cynomolgus)
UniProt Name IL10_HUMAN (Human)
IL10_MACFA (Cynomolgus)

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  1. 1. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut)
  2. 2. Gotlieb WH, Abrams JS, Watson JM, Velu TJ, Berek JS, Martínez-Maza O. Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancers. Cytokine. 1992;4:385-90. (ELISA-Detection, Viral Reactivity)
  3. 3. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
  4. 4. Kawamoto S, Nitta Y, Tashiro F, Nakano A, Yamato E, Tahara H, et al. Suppression of Th1 cell activation and prevention of autoimmune diabetes in NOD mice by local expression of viral IL-10. Int Immunol. 2001;13:685-94. (ELISA-Detection, WB, Viral Reactivity)
  5. 5. Hansson T, Dannæus A, Klareskog L. Cytokine-producing cells in peripheral blood of children with coeliac disease secrete cytokines with a type 1 profile. Clin Exp Immunol. 1999;116:246-50. (ELISPOT-Detection)
  6. 6. Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, et al. Detection of low-frequency antigen-specific IL-10-producing CD4+ T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J Immunol Methods. 2003;279:111-21. (ELISA-Detection, ELISPOT-Detection)
  7. 7. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Detection)
  8. 8. Andersson J, Abrams J, Björk L, Funa K, Litton M, Ågren K, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994;83:16-24. (IHC-FS)
  9. 9. Ulfgren A, Lindblad S, Klareskog L, Andersson J, Andersson U. Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis. Ann Rheum Dis. 1995;54:654-61. (IHC-FS)
  10. 10. Bergeron A, Soler P, Kambouchner M, Loiseau P, Milleron B, Valeyre D, et al. Cytokine profiles in idiopathic pulmonary fibrosis suggest an important role for TGF-β and IL-10. Eur Respir J. 2003;22:69-76. (IHC-FS)
  11. 11. Bellone G, Carbone A, Smirne C, Scirelli T, Buffolino A, Novarino A, et al. Cooperative induction of a tolerogenic dendritic cell phenotype by cytokines secreted by pancreatic carcinoma cells. J Immunol. 2006;177:3448-60. (IHC-PS)
  12. 12. Brodeur ND, Spencer JV. Antibodies to human IL-10 neutralize ebvIL-10-mediated cytokine suppression but have no effect on cmvIL-10 activity. Virus Res. 2010;153:265-8. (WB, Neut, Viral Reactivity)
  13. 13. Groux H, O'Garra A, Bigler M, Rouleau M, Antonenko S, de Vries JE, et al. A CD4+ T-cell subset inhibits antigen-specific T-cell responses and prevents colitis. Nature. 1997;389:737-42. (Neut)
  14. 14. Hutchinson KL, Villinger F, Miranda ME, Ksiazek TG, Peters CJ, Rollin PE. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6. (Multiplex-Detection, Cynomolgus Reactivity)
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