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Rat Anti-Mouse BiP-BIOT (76-E6)

Cat. No.:
Biotin Anti-Mouse BiP antibody for use in ELISA, western blot, and immunoprecipitation assays.
Size Price (USD) Quantity
0.5 mg $336.00
More Information
Clone 76-E6
Isotype Rat (Fischer) IgG2aκ
Isotype Control Rat IgG2a-BIOT (KLH/G2a-1-1)
Specificity Mouse BiP
Alternative Names GRP78
Description The immunoglobulin heavy chain binding protein BiP is a member of the hsp70 family of heat shock proteins and is identical to the glucose regulated protein GRP78. While BiP was originally described for its function in B cells, it is now known to be distributed in a variety of tissues, if not ubiquitous. The highly conserved hsp70 proteins have an essential physiological role in stress responses and as “molecular chaperones” which are responsible for a variety of functions such as protein transport, prevention of protein toxicity and direction of protein folding. With regard to its immunological role, BiP is a component of the endoplasmic reticulum and binds free intracellular heavy chains in nonsecreting pre-B cell lines (μ+,L-) or incompletely assembled Ig precursors in H+L+ secreting hybridomas and myelomas. In the absence of light chain synthesis, heavy chains remain associated with BiP and are not secreted. BiP is an ATP binding protein and the dissociation of the BiP-heavy chain complex is probably driven by the ATPase activity attributed to BiP. The monoclonal antibody 76-E6 recognizes a conserved epitope localized within the region of amino acids 497 to 581 of BiP.
Immunogen Mouse BiP:μ chain complexes
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Western Blot – Quality tested 2,3
Immunoprecipitation – Reported in literature 1
ELISA – Reported in literature 1

RRID Number AB_2795269
Gene ID 14828 (Mouse)
Gene ID Symbol Hspa5 (Mouse)
Gene ID Aliases Bip; Sez7; mBiP; Grp78; SEZ-7; Hsce70; baffled; AL022860; AU019543; D2Wsu17e; D2Wsu141e
UniProt ID P20029 (Mouse)
UniProt Name BIP_MOUSE (Mouse)

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  1. 1. Bole DG, Hendershot LM, Kearney JF. Posttranslational association of immunoglobulin heavy chain binding protein with nascent heavy chains in nonsecreting and secreting hybridomas. J Cell Biol. 1986;102:1558-66. (Immunogen, ELISA, IP)
  2. 2. Elkabetz Y, Argon Y, Bar-Nun S. Cysteines in CH1 underlie retention of unassembled Ig heavy chains. J Biol Chem. 2005;280:14402-12. (WB)
  3. 3. Elkabetz Y, Shapira I, Rabinovich E, Bar-Nun S. Distinct steps in dislocation of luminal endoplasmic reticulum-associated degradation substrates: roles of endoplamic reticulum-bound p97/Cdc48p and proteasome. J Biol Chem. 2004;279:3980-89. (WB)