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  • Sheep Anti-Rat IgG(H+L)-BIOT

Sheep Anti-Rat IgG(H+L)-BIOT

Cat. No.:
6900-08
Sheep Anti-Rat IgG(H+L)-Biotin antibody for use in ELISA assays.
$76.00
Size Price (USD) Quantity
1.0 mg $76.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Sheep IgG
Isotype Control Sheep IgG-BIOT
Specificity Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM
Source Pooled antisera from sheep hyperimmunized with rat IgG
Cross Adsorption None; may react with immunoglobulins from other species and the light chains of other rat immunoglobulins
Purification Method Affinity chromatography on rat IgG covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA

RRID Number AB_2895840

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. NIH/Swiss mouse fibroblast cell line 3T3 was stained with Rat Anti-β-Actin-UNLB (right) followed by Donkey Anti-Rat IgG(H+L), Mouse SP ads-BIOT (SB Cat. No. 6430-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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  2. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45-UNLB (SB Cat. No. 1660-01) followed by Goat Anti-Rat Ig, Mouse ads-BIOT (SB Cat. No. 3010-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
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  4. Frozen BALB/c mouse intestine tissue was stained with Rat IgG<sub>2a</sub>-UNLB isotype control (SB Cat. No. 0117-01; left) and Rat Anti-Mouse CD104-UNLB (SB Cat. No. 1855-01; right) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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  5. Paraffin embedded mouse small intestine tissue section was blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Rat Anti-Mouse IgA-UNLB (SB Cat. No. 1165-01) followed by Rabbit Anti-Rat IgG(H+L), Human ads-BIOT (SB Cat. No. 6185-08), Streptavidin-FITC (SB Cat. No. 7100-02), DAPI, and mounted with Fluoromount-G<sup>&reg;</sup> Anti-Fade (SB Cat. No. 0100-35).
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  6. Frozen rat gastrocnemic muscle section was stained with Goat Anti-Rat IgM-BIOT (SB Cat. No. 3020-08) followed by a CY3 secondary reagent and DAPI.<br/>Image from Duehrkop C, Banz Y, Spirig R, Miescher S, Nolte MW, Spycher M, et al. C1 esterase inhibitor reduces lower extremity ischemia/reperfusion injury and associated lung damage. PLoS One. 2013;8(8):e72059. Figure 2(h)<br/>Reproduced under the Creative Commons Attribution License
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  7. ELISA plate was coated with serially diluted Rat IgG-UNLB (SB Cat. No. 0108-01). Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) and Mouse Anti-Rat IgM-BIOT (SB Cat. No. 3080-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  8. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with Rabbit Anti-Rat IgG(H+L)-BIOT (SB Cat. No. 6180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  9. ELISA plate was coated with purified rat IgG and IgM, mouse IgG, IgM, and IgA, and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG(H+L), Mouse/Human ads-UNLB (SB Cat. No. 3051-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  10. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Rabbit F(ab')<sub>2</sub> Anti-Rat IgG(H+L)-BIOT (SB Cat. No. 6130-08) followed by Streptavidin-HRP (SB Cat. No. 7105-05).
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  11. ELISA plate was coated with serially diluted Rat IgM-UNLB (SB Cat. No. 0120-01). Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) and Goat Anti-Rat IgM-BIOT (SB Cat. No. 3020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  12. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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