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Mouse IgM-PE/CY5.5

Cat. No.
0101-16  
Size
100 tests  
Price (USD)
$160.00 
Qty
Add to Cart
Clone 11E10
Isotype Mouse IgMλ
Specificity Lipopolysaccharide
Format/Conjugate PE/CY5.5 (R-phycoerythrin/Cyanine 5.5)
Buffer Formulation Supplied in PBS/NaN3 and a stabilizing agent
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 17-19
ELISA – Quality tested 1-13
FLISA – Quality tested
Immunohistochemistry-Frozen Sections – Reported in literature 14,15
Immunohistochemistry-Paraffin Sections – Reported in literature 16
Blocking – Reported in literature 6
In vitro Control – Reported in literature 20-22
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with Goat Anti-Mouse IgG, Human ads-UNLB (SB Cat. No. 1030-01), Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01), and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01). Serially diluted Mouse IgM-BIOT (SB Cat. No. 0101-08) was captured followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.

ELISA plate was coated with Goat Anti-Mouse IgG, Human ads-UNLB (SB Cat. No. 1030-01), Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01), and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01). Serially diluted Mouse IgM-BIOT (SB Cat. No. 0101-08) was captured followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
 
References
1. Oliver AM, Martin F, Kearney JF. IgMhighCD21high lymphocytes enriched in the splenic marginal zone generate effector cells more rapidly than the bulk of follicular B cells. J Immunol. 1999;162:7198-207. (ELISA)
2. Beger E, Deocharan B, Edelman M, Erblich B, Gu Y, Putterman C. A peptide DNA surrogate accelerates autoimmune manifestations and nephritis in lupus-prone mice. J Immunol. 2002;168:3617-26. (ELISA)
3. Aït-Azzouzene D, Verkoczy L, Duong B, Skog P, Gavin AL, Nemazee D. Split tolerance in peripheral B cell subsets in mice expressing a low level of Igκ-reactive ligand. J Immunol. 2006;176:939-48. (ELISA)
4. Genestier L, Taillardet M, Mondiere P, Gheit H, Bella C, Defrance T. TLR agonists selectively promote terminal plasma cell differentiation of B cell subsets specialized in thymus-independent responses. J Immunol. 2007;178:7779-86. (ELISA)
5. Wang Y, Diamond B. B cell receptor revision diminishes the autoreactive B cell response after antigen activation in mice. J Clin Invest. 2008;118:2896-907. (ELISA)
6. Huang L, Adachi T, Shimizu Y, Goto Y, Toyama J, Tanaka H, et al. Characterization of lectin isolated from Momordica charantia seed as a B cell activator. Immunol Lett. 2008;121:148-56. (ELISA, Block)
7. Côrte-Real J, Rodo J, Almeida P, Garcia J, Coutinho A, Demengeot J, et al. Irf4 is a positional and functional candidate gene for the control of serum IgM levels in the mouse. Genes Immun. 2009;10:93-9. (ELISA)
8. Patterson HC, Kraus M, Wang D, Shahsafaei A, Henderson JM, Seagal J, et al. Cytoplasmic Igα serine/threonines fine-tune Ig α tyrosine phosphorylation and limit bone marrow plasma cell formation. J Immunol. 2011;187:2853-8. (ELISA)
9. Szymczak WA, Davis MJ, Lundy SK, Dufaud C, Olszewski M, Pirofski L. X-linked immunodeficient mice exhibit enhanced susceptibility to Cryptococcus neoformans infection. Mbio. 2013;4(4): e00265-13. (ELISA)
10. Alsadeq A, Hobeika E, Medgyesi D, Kläsener K, Reth M. The role of the Syk/Shp-1 kinase-phosphatase equilibrium in B cell development and signaling. J Immunol. 2014;193:268-76. (ELISA)
11. Rosenbaum M, Andreani V, Kapoor T, Herp S, Flach H, Duchniewicz M, et al. MZB1 is a GRP94 cochaperone that enables proper immunoglobulin heavy chain biosynthesis upon ER stress. Genes Dev. 2014;28:1165-78. (ELISA)
12. Vogelzang A, McGuire HM, Liu SM, Gloss B, Mercado K, Earls P, et al. IL-21 contributes to fatal inflammatory disease in the absence of Foxp3+ T regulatory cells. J Immunol. 2014;192:1404-14. (ELISA)
13. John S, Russell L, Chin SS, Luo W, Oshima R, Garrett-Sinha LA. Transcription factor Ets1, but not the closely related factor Ets2, inhibits antibody-secreting cell differentiation. Mol Cell Biol. 2014;34:522-32. (ELISA)
14. Nesse W, Westra J, van der Wal JE, Abbas F, Nicholas AP, Vissink A, et al. The periodontium of periodontitis patients contains citrullinated proteins which may play a role in ACPA (anti-citrullinated protein antibody) formation. J Clin Periodontol. 2012;39:599-607. (IHC-FS)
15. Kiekens RC, Thepen T, Bihari IC, Knol EF, Van De Winkel JG, Bruijnzeel-Koomen CA. Expression of Fc receptors for IgG during acute and chronic cutaneous inflammation in atopic dermatitis. Br J Dermatol. 2000;142:1106-13. (IHC-FS)
16. Dadachova E, Nosanchuk JD, Shi L, Schweitzer AD, Frenkel A, Nosanchuk JS, et al. Dead cells in melanoma tumors provide abundant antigen for targeted delivery of ionizing radiation by a mAb to melanin. Proc Natl Acad Sci USA. 2004;101:14865-70. (IHC-PS)
17. Mierke CT, Ballmaier M, Werner U, Manns MP, Welte K, Bischoff SC. Human endothelial cells regulate survival and proliferation of human mast cells. J Exp Med. 2000;192:801-11. (FC)
18. Cheng J, Hylander BL, Baer MR, Chen X, Repasky EA. Multiple mechanisms underlie resistance of leukemia cells to Apo2 Ligand/TRAIL. Mol Cancer Ther. 2006;5:1844-53. (FC)
19. Nguyen TT, Elsner RA, Baumgarth N. Natural IgM prevents autoimmunity by enforcing B cell central tolerance induction. J Immunol. 2015;194:1489-1502. (FC)
20. Pedersen ED, Aass HC, Rootwelt T, Fung M, Lambris JD, Mollnes TE. CD59 efficiently protects human NT2-N neurons against complement-mediated damage. Scand J Immunol. 2007;66:345-51. (In vitro control)
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