Cat. No.
0109-07

Size
100 tests

Price (USD)
$30.00

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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Source	Normal goat serum
Format/Conjugate	TXRD (Texas Red^®)
Buffer Formulation	Supplied in PBS/NaN₃
Volume	1.0 mL
Storage & Handling	Store at 2-8°C Please refer to product specific SDS
Applications	Quality tested applications for relevant formats include - Flow Cytometry ^1-4 ELISA FLISA Other referenced applications for relevant formats include - Immunohistochemistry-Frozen Sections ^5-7 Immunohistochemistry-Paraffin Sections ⁸ Immunohistochemistry-Whole Mount ¹⁸ Immunocytochemistry ^19,20 Immunoprecipitation ²¹ Blocking ^4,9,10 In vitro Control ^11-15 In vivo Control ^16,17
Recommended Dilutions	Please refer to product specific Technical Bulletin

Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.

Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.

References

1.	Harriman GR, Kunimoto DY, Elliott JF, Paetkau V, Strober W. The role of IL-5 in IgA B cell differentiation. J Immunol. 1988;140:3033-39. (FC)
2.	Pospisil R, Young-Cooper GO, Mage RG. Preferential expansion and survival of B lymphocytes based on VH framework 1 and framework 3 expression: "positive" selection in appendix of normal and V_H-mutant rabbits. Proc Natl Acad Sci USA. 1995;92:6961-5. (FC)
3.	Milpied P, Renard A, Bruneau J, Mendes-da-Cruz DA, Jacquelin S, Asnafi V, et al. Neuropilin-1 is not a marker of human Foxp3⁺ Treg. Eur J Immunol. 2009;39:1466-71. (FC)
4.	Saiya-Cork K, Collins R, Parkin B, Ouillette P, Kuizon E, Kujawski L, et al. A pathobiological role of the insulin receptor in chronic lymphocytic leukemia. Clin Cancer Res. 2011;17:2679-92. (FC, Block)
5.	Sleeman MA, Watson JD, Murison JG. Neonatal murine epidermal cells express a functional multidrug-resistant pump. J Invest Dermatol. 2000;115:19-23. (IHC-FS)
6.	Annunziato F, Cosmi L, Liotta F, Lazzeri E, Manetti R, Vanini V, et al. Phenotype, localization, and mechanism of suppression of CD4⁺CD25⁺ human thymocytes. J Exp Med. 2002;196:379-87. (IHC-FS)
7.	Menke J, Zeller GC, Kikawada E, Means TK, Huang XR, Lan HY, et al. CXCL9, but not CXCL10, promotes CXCR3-dependent immune-mediated kidney disease. J Am Soc Nephrol. 2008;19:1177-89. (IHC-FS)
8.	Tamura M, Aizawa R, Hori M, Ozaki H. Progressive renal dysfunction and macrophage infiltration in interstitial fibrosis in an adenine-induced tubulointerstitial nephritis mouse model. Histochem Cell Biol. 2009;131:483-90. (IHC-PS)
9.	Boesze-Battaglia K, Besack D, McKay T, Zekavat A, Otis L, Jordan-Sciutto K, et al. Cholesterol-rich membrane microdomains mediate cell cycle arrest induced by Actinobacillus actinomycetemcomitans cytolethal-distending toxin. Cell Microbiol. 2006;8:823-36. (Block)
10.	Pepin AC, Tandon R, Cattori V, Niederer E, Riond B, Willi B, et al. Cellular segregation of feline leukemia provirus and viral RNA in leukocyte subsets of long-term experimentally infected cats. Virus Res. 2007;127:9-16. (Block)
11.	Mierke CT, Ballmaier M, Werner U, Manns MP, Welte K, Bischoff SC. Human endothelial cells regulate survival and proliferation of human mast cells. J Exp Med. 2000;192:801-11. (In vitro control)
12.	Sato H, Wang D, Kudo A. Dissociation of Pax-5 from KI and KII sites during κ-chain gene rearrangement correlates with its association with the underphosphorylated form of retinoblastoma. J Immunol. 2001;166:6704-10. (In vitro control)
13.	Petlickovski A, Laurenti L, Li X, Marietti S, Chiusolo P, Sica S, et al. Sustained signaling through the B-cell receptor induces Mcl-1 and promotes survival of chronic lymphocytic leukemia B cells. Blood. 2005;105:4820-7. (In vitro control)
14.	Zizzo G, Hilliard BA, Monestier M, Cohen PL. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20. (In vitro control)
15.	Li H, Usas A, Poddar M, Chen C, Thompson S, Ahani B, et al. Platelet-rich plasma promotes the proliferation of human muscle derived progenitor cells and maintains their stemness. PLoS One. 2013;8(6):e64923. (In vitro control)
16.	Lo JC, Chin RK, Lee Y, Kang H, Wang Y, Weinstock JV, et al. Differential regulation of CCL21 in lymphoid/nonlymphoid tissues for effectively attracting T cells to peripheral tissues. J Clin Invest. 2003;112:1495-505. (In vivo control)
17.	Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI. The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1β. Genes Dev. 2006;20:2527-38. (In vivo control)
18.	Lämmermann T, Afonso PV, Angermann BR, Wang JM, Kastenmüller W, Parent CA, et al. Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature. 2013;498:371-5. (IHC-WM)
19.	Hauser SP, Waldron JA, Upuda KB, Lipschitz DA. Morphological characterization of stromal cell types in hematopoietically active long-term murine bone marrow cultures. J Histochem Cytochem. 1995;43:371-9. (ICC)
20.	Hsieh-Bonassera ND, Wu I, Lin JK, Schumacher BL, Chen AC, Masuda K, et al. Expansion and redifferentiation of chondrocytes from osteoarthritic cartilage: cells for human cartilage tissue engineering. Tissue Eng Part A. 2009;15:3513-23. (ICC)
21.	Kishimoto N, Onitsuka A, Kido K, Takamune N, Shoji S, Misumi S. Glyceraldehyde 3-phosphate dehydrogenase negatively regulates human immunodeficiency virus type 1 infection. Retrovirology. 2012;9:107. (IP)

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Goat IgG-TXRD