Cat. No.
0117-11

Size
0.1 mg

Price (USD)
$167.00

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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Clone	KLH/G2a-1-1
Isotype	Rat IgG_2aκ
Specificity	KLH
Format/Conjugate	APC (Allophycocyanin)
Buffer Formulation	Supplied in PBS/NaN₃ and a stabilizing agent
Concentration	0.1 mg/mL
Volume	1.0 mL
Storage & Handling	Store at 2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - Flow Cytometry – Quality tested ^1-7 ELISA – Quality tested FLISA – Quality tested Immunohistochemistry-Frozen Sections – Reported in literature ⁸ Immunohistochemistry-Paraffin Sections ¹⁴ Immunoprecipitation – Reported in literature ⁹ Blocking – Reported in literature ¹⁰ In vitro Control – Reported in literature ¹¹ In vivo Control – Reported in literature ^12,13
Recommended Dilutions	Please refer to product specific Technical Bulletin

FLISA plate was coated with Mouse Anti-Rat IgG1-UNLB (SB Cat. No. 3061-01), Mouse Anti-Rat IgG2a-UNLB (SB Cat. No. 3065-01), Mouse Anti-Rat IgG2b-UNLB (SB Cat. No. 3070-01), Mouse Anti-Rat IgG2c-UNLB (SB Cat. No. 3075-01), and Mouse Anti-Rat IgM-UNLB (SB Cat. No. 3080-01). Serially diluted Rat IgG2a-APC (SB Cat. No. 0117-11) was captured and fluorescence intensity quantified.

FLISA plate was coated with Mouse Anti-Rat IgG1-UNLB (SB Cat. No. 3061-01), Mouse Anti-Rat IgG2a-UNLB (SB Cat. No. 3065-01), Mouse Anti-Rat IgG2b-UNLB (SB Cat. No. 3070-01), Mouse Anti-Rat IgG2c-UNLB (SB Cat. No. 3075-01), and Mouse Anti-Rat IgM-UNLB (SB Cat. No. 3080-01). Serially diluted Rat IgG2a-APC (SB Cat. No. 0117-11) was captured and fluorescence intensity quantified.

References

1.	Welner R, Swett DJ, Pelsue SC. Age-related loss of bone marrow pre-B- and immature B-lymphocytes in the autoimmune-prone flaky skin mutant mice. Autoimmunity. 2005;38:399-408. (FC)
2.	Gillan V, Devaney E. Regulatory T cells modulate Th2 responses induced by Brugia pahangi third-stage larvae. Infect Immun. 2005;73:4034-42. (FC)
3.	Friesen C, Glatting G, Koop B, Schwarz K, Morgenstern A, Apostolidis C, et al. Breaking chemoresistance and radioresistance with [213Bi]anti-CD45 antibodies in leukemia cells. Cancer Res. 2007;67:1950-8. (FC)
4.	Gillan V, Lawrence RA, Devaney E. B cells play a regulatory role in mice infected with the L3 of Brugia pahangi. Int Immunol. 2005;17:373-82. (FC)
5.	Chu VT, Fröhlich A, Steinhauser G, Scheel T, Roch T, Fillatreau S, et al. Eosinophils are required for the maintenance of plasma cells in the bone marrow. Nat Immunol. 2011;12:151-60. (FC)
6.	Benhamron S, Reiner I, Zcharia E, Atallah M, Grau A, Vlodavsky I, et al. Dissociation between mature phenotype and impaired transmigration in dendritic cells from heparanase-deficient mice. PLoS One. 2012;7(5):e35602. (FC)
7.	Berge T, Grønningsæter IH, Lorvik KB, Abrahamsen G, Granum S, Sundvold-Gjerstad V, et al. SH2D2A modulates T cell mediated protection to a B cell derived tumor in transgenic mice. PLoS One. 2012;7(10):e48239. (FC)
8.	Løvås T, Bruusgaard JC, Øynebråten I, Gundersen K, Bogen B. DNA vaccines: MHC II-targeted vaccine protein produced by transfected muscle fibres induces a local inflammatory cell infiltrate in mice. PLoS One. 2014;9(10):e108069. (IHC-FS)
9.	Rosenbaum M, Andreani V, Kapoor T, Herp S, Flach H, Duchniewicz M, et al. MZB1 is a GRP94 cochaperone that enables proper immunoglobulin heavy chain biosynthesis upon ER stress. Genes Dev. 2014;28:1165-78. (IP)
10.	Talkington J, Nickell SP. Role of Fc gamma receptors in triggering host cell activation and cytokine release by Borrelia burgdorferi. Infect Immun. 2001;69:413-9. (Block)
11.	Mayo L, Jacob-Hirsch J, Amariglio N, Rechavi G, Moutin M, Lund FE, et al. Dual role of CD38 in microglial activation and activation-induced cell death. J Immunol. 2008;181:92-103. (In vitro control)
12.	Kaplan RN, Riba RD, Zacharoulis S, Bramley AH, Vincent L, Costa C, et al. VEGFR1-positive haematopoietic bone marrow progenitors initiate the pre-metastatic niche. Nature. 2005;438:820-7. (In vivo control)
13.	Etich J, Bergmeier V, Frie C, Kreft S, Bengestrate L, Eming S, et al. PECAM1⁺/Sca1⁺/CD38⁺ vascular cells transform into myofibroblast-like cells in skin wound repair. PloS One. 2013;8(1):e53262. (In vivo control)
14.	SouthernBiotech unpublished data (IHC-PS)

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