FLISA plate was coated with Mouse Anti-Rat IgG1-UNLB (SB Cat. No. 3061-01), Mouse Anti-Rat IgG2a-UNLB (SB Cat. No. 3065-01), Mouse Anti-Rat IgG2b-UNLB (SB Cat. No. 3070-01), Mouse Anti-Rat IgG2c-UNLB (SB Cat. No. 3075-01), and Mouse Anti-Rat IgM-UNLB (SB Cat. No. 3080-01). Serially diluted Rat IgG2c-AF488 (SB Cat. No. 0119-30) was captured and fluorescence intensity quantified.