Sheep IgG-BIOT

Cat. No.
0133-08  
Size
1.0 mg  
Price (USD)
$50.00 
Source Normal sheep serum
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 2.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
Flow Cytometry
ELISA
FLISA
Other referenced applications for relevant formats include -
Immunocytochemistry 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794071
  • BALB/c mouse splenocytes were stained with Sheep IgG-BIOT (SB Cat. No. 0133-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
  • ELISA plate was coated with Rabbit Anti-Sheep IgG(H+L)-UNLB (SB Cat. No. 6150-01). Serially diluted Sheep IgG-BIOT (SB Cat. No. 0133-08) was captured followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
  • Human peripheral blood lymphocytes were stained with Sheep IgG-BIOT (SB Cat No. 0133-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).

BALB/c mouse splenocytes were stained with Sheep IgG-BIOT (SB Cat. No. 0133-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
 

ELISA plate was coated with Rabbit Anti-Sheep IgG(H+L)-UNLB (SB Cat. No. 6150-01). Serially diluted Sheep IgG-BIOT (SB Cat. No. 0133-08) was captured followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
 

Human peripheral blood lymphocytes were stained with Sheep IgG-BIOT (SB Cat No. 0133-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
 
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References
1. Chimen M, McGettrick HM, Apta B, Kuravi SJ, Yates CM, Kennedy A, et al. Homeostatic regulation of T cell trafficking by a B cell-derived peptide is impaired in autoimmune and chronic inflammatory disease. Nat Med. 2015;21:467-75. (ICC)