Unlabeled Anti-Bcl-xL, 7B2.5

Cat. No.
10030-01

Size
0.1 mg

Price (USD)
$325.00

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Technical Bulletin Certificate of
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Clone	7B2.5
Isotype	Mouse (BALB/c) IgG₃κ
Isotype Control	Mouse IgG₃-UNLB
Immunogen	Recombinant human Bcl-x_S
Specificity	Human/Mouse/Rat/Rhesus Bcl-x_L
Alternalte Name(s)	Apoptosis regulator Bcl-X, Bcl-2-like 1 protein, BCL2L1
Description	Bcl-x_L is a 29 kDa member of the Bcl-2 family of proteins involved in regulation of programmed cell death, or apoptosis. It is found in both soluble and membrane fractions of cellular lysates. Like Bcl-2, Bcl-x_L has been demonstrated to block apoptosis which is induced by a variety of stimuli and, under certain conditions, offers greater protection against apoptosis than Bcl-2. While formation of heterodimers with the apoptosis-enhancing protein, Bax does not block the apoptosis-suppressing property of Bcl-x_L, heterodimerization with Bad effectively inhibits the protective function of Bcl-x_L.
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Borate buffered saline, pH 8.2
Concentration	0.1 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - Flow Cytometry – Quality tested ^2-25 Immunohistochemistry-Paraffin Sections – Reported in literature ²⁶ Immunocytochemistry – Reported in literature ²⁷ Western Blot – Reported in literature ^1,28-30 Immunoprecipitation – Reported in literature ^30,31 ELISA – Reported in literature ¹
Recommended Dilutions	Please refer to product specific Technical Bulletin

Human T cell leukemia cell line Jurkat was intracellularly stained with Mouse Anti-Human Bcl-xL-FITC (SB Cat. No. 10030-02).

Human T cell leukemia cell line Jurkat was intracellularly stained with Mouse Anti-Human Bcl-xL-FITC (SB Cat. No. 10030-02).

References

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3.	Homann D, Teyton L, Oldstone MB. Differential regulation of antiviral T-cell immunity results in stable CD8⁺ but declining CD4⁺ T-cell memory. Nat Med. 2001;7:913-9. (FC)
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18.	Osborne LC, Duthie KA, Seo JH, Gascoyne RD, Abraham N. Selective ablation of the YxxM motif of IL-7Rα suppresses lymphomagenesis but maintains lymphocyte development. Oncogene. 2010;29:3854-64. (FC)
19.	Chang C, Lai Y, Hou M, Huang P, Liao N. IL-15Rα of radiation-resistant cells is necessary and sufficient for thymic invariant NKT cell survival and functional maturation. J Immunol. 2011;187:1235-42. (FC)
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Mouse Anti-Bcl-xL-UNLB

Mouse Anti-Bcl-x_L-UNLB