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Mouse Anti-Bcl-xL-UNLB

Cat. No.
10035-01  
Size
0.1 mg  
Price (USD)
$185.00 
Qty
Add to Cart
Clone 2H12
Isotype Mouse IgG2aκ
Isotype Control Mouse IgG2a-UNLB
Immunogen Bcl-xL peptide (a.a. 3-14) common to mouse and human
Specificity Mouse/Rat/Human Bcl-xL
Alternalte Name(s) Apoptosis regulator Bcl-X, Bcl-2-like 1 protein, BCL2L1
Description Bcl-xL is a 29 kDa member of the Bcl-2 family of proteins that has been shown to block apoptosis. The monoclonal antibody 2H12 reacts with an N-terminal epitope between amino acids 3-14 that is common to mouse, human, and rat Bcl-xL.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Immunoblotting – Quality tested 1-7
Immunoprecipitation – Reported in literature 6,8
Flow Cytometry – Reported in literature 5,7,9
Immunohistochemistry-Paraffin Sections – Reported in literature 4,10,11
ELISA – Reported in literature 12
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Total cell lysates from mouse thymocytes and Hep G2 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Bcl-xL-UNLB (SB Cat. No. 10035-01). Proteins were visualized using Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05) secondary antibody and chemiluminescent detection.

Total cell lysates from mouse thymocytes and Hep G2 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Bcl-xL-UNLB (SB Cat. No. 10035-01). Proteins were visualized using Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05) secondary antibody and chemiluminescent detection.
 
References
1. Hsu Y, Youle RJ. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 1997;272:13829-34. (Immunogen, WB)
2. Hsu Y, Wolter KG, Youle RJ. Cytosol-to-membrane redistribution of Bax and Bcl-XL during apoptosis. Proc Natl Acad Sci USA. 1997;94:3668-72. (WB)
3. Wolter KG, Hsu Y, Smith CL, Nechushtan A, Xi X, Youle RJ. Movement of Bax from the cytosol to mitochondria during apoptosis. J Cell Biol. 1997;139:1281-92. (WB)
4. Chu W, Aguilera NS, Wei MQ, Abbondanzo SL. Antiapoptotic marker Bcl-XL, expression on Reed-Sternberg cells of Hodgkin's disease using a novel monoclonal marker, YTH-2H12. Hum Pathol. 1999;30:1065-70. (WB, IHC-PS)
5. Ferrero E, Belloni D, Contini P, Foglieni C, Ferrero ME, Fabbri M, et al. Transendothelial migration leads to protection from starvation-induced apoptosis in CD34+CD14+ circulating precursors: evidence for PECAM-1 involvement through Akt/PKB activation. Blood. 2003;101:186-93. (WB, FC)
6. Jeong S, Gaume B, Lee Y, Hsu Y, Ryu S, Yoon S, et al. Bcl-xL sequesters its C-terminal membrane anchor in soluble, cytosolic homodimers. EMBO J. 2004;23:2146-55. (WB, IP)
7. Dodson LF, Boomer JS, Deppong CM, Shah DD, Sim J, Bricker TL, et al. Targeted knock-in mice expressing mutations of CD28 reveal an essential pathway for costimulation. Mol Cell Biol. 2009;29:3710-21. (WB, FC)
8. Pérez-Galán P, Roué G, Villamor N, Campo E, Colomer D. The BH3-mimetic GX15-070 synergizes with bortezomib in mantle cell lymphoma by enhancing Noxa-mediated activation of Bak. Blood. 2007;109:4441-9. (IP)
9. Boomer JS, Deppong CM, Shah DD, Bricker TL, Green JM. Cutting edge: A double-mutant knockin of the CD28 YMNM and PYAP motifs reveals a critical role for the YMNM motif in regulation of T cell proliferation and Bcl-xL expression. J Immunol. 2014;192:3465-9. (FC)
10. Hyjek E, Chadburn A, Liu YF, Cesarman E, Knowles DM. BCL-6 protein is expressed in precursor T-cell lymphoblastic lymphoma and in prenatal and postnatal thymus. Blood. 2011;97:270-6. (IHC-PS)
11. Aebersold DM, Kollar A, Beer KT, Laissue J, Greiner RH, Djonoz V. Involvement of the hepatocyte growth factor/scatter factor receptor c-met and of Bcl-xL in the resistance of oropharyngeal cancer to ionizing radiation. Int J Cancer. 2001;96:41-54. (IHC-PS)
12. Hackett GS. Intracellular delivery of therapeutic antibodies [dissertation]. Nottingham (United Kingdom): University of Nottingham; 2011. (ELISA)
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