Unlabeled ApoScreen® Annexin V

Cat. No.
10039-01

Size
0.1 mg

Price (USD)
$185.00

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Technical Bulletin Certificate of
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Description	ApoScreen^® Annexin V (SB Cat. No. 10039) is a recombinant protein that is expressed in eukaryotic cells. A number of changes in cell structure occur during apoptosis. One major change is the loss of membrane phospholipid asymmetry, with translocation of phosphatidylserine (PS) from the inner leaflet of the phospholipid bilayer to the external surface. Annexin V, a member of the annexin family of calcium-dependent phospholipid binding proteins, has a high affinity for phosphatidylserine (PS)-containing phospholipid bilayers. Conjugated Annexin V can be used to monitor changes in cell membrane phospholipid asymmetry, thereby providing a convenient tool for detection of apoptotic cells.
Source	Eukaryotic cells
Buffer Formulation	Supplied lyophilized from phosphate buffered saline and 1 mM EDTA
Storage & Handling	Store at 2-8°C Please refer to product specific SDS
Applications	Flow Cytometry – Quality tested ¹ Immunocytochemistry ²
Recommended Dilutions	Please refer to product specific Technical Bulletin

Day old BALB/c mouse splenocytes were stained with Annexin V-BIOT (SB Cat. No. 10039-08) with and without Propidium Iodide (SB Cat. No. 10044-01) followed by Streptavidin-FITC (SB Cat. No. 7100-02).

Day old BALB/c mouse splenocytes were stained with Annexin V-BIOT (SB Cat. No. 10039-08) with and without Propidium Iodide (SB Cat. No. 10044-01) followed by Streptavidin-FITC (SB Cat. No. 7100-02).

References

1.	Peterson LF, Mitrikeska E, Giannola D, Lui Y, Sun H, Bixby D, et al. p53 stabilization induces apoptosis in chronic myeloid leukemia blast crisis cells. Leukemia. 2011;25:761-9. (FC)
2.	SouthernBiotech published data (ICC)

ApoScreen^® is a registered trademark of SouthernBiotechnology Associates, Inc.

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ApoScreen® Annexin V-UNLB

ApoScreen^® Annexin V-UNLB