ApoScreen® Annexin V-FITC

Cat. No.
10039-02  
Size
100 tests  
Price (USD)
$195.00 
Description ApoScreen® Annexin V (SB Cat. No. 10039) is a recombinant protein that is expressed in eukaryotic cells. A number of changes in cell structure occur during apoptosis. One major change is the loss of membrane phospholipid asymmetry, with translocation of phosphatidylserine (PS) from the inner leaflet of the phospholipid bilayer to the external surface. Annexin V, a member of the annexin family of calcium-dependent phospholipid binding proteins, has a high affinity for phosphatidylserine (PS)-containing phospholipid bilayers. Conjugated Annexin V can be used to monitor changes in cell membrane phospholipid asymmetry, thereby providing a convenient tool for detection of apoptotic cells.
Source Eukaryotic cells
Buffer Formulation Supplied in ApoScreen® Annexin V Binding Buffer
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications Flow Cytometry – Quality tested 1
Immunocytochemistry 2
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Human T lymphocyte cell line Jurkat was stained with ApoScreen® Annexin V-FITC (SB Cat No. 10039-02) and ApoScreen® 7-Aminoactinomycin D (7-AAD; SB Cat. No. 10042-01) at varying stages of apoptosis. (Green - Annexin V-FITC; 7-AAD - Red)

Human T lymphocyte cell line Jurkat was stained with ApoScreen® Annexin V-FITC (SB Cat No. 10039-02) and ApoScreen® 7-Aminoactinomycin D (7-AAD; SB Cat. No. 10042-01) at varying stages of apoptosis. (Green - Annexin V-FITC; 7-AAD - Red)
 
References
1. Peterson LF, Mitrikeska E, Giannola D, Lui Y, Sun H, Bixby D, et al. p53 stabilization induces apoptosis in chronic myeloid leukemia blast crisis cells. Leukemia. 2011;25:761-9. (FC)
2. SouthernBiotech published data (ICC)
ApoScreen® is a registered trademark of SouthernBiotechnology Associates, Inc.