The Antibody Company - SouthernBiotech
 
   

Mouse Anti-Mouse Bax-UNLB

Cat. No.
10050-01  
Size
0.1 mg  
Price (USD)
$180.00 
Qty
Add to Cart
Clone 5B7
Isotype Mouse IgG1κ
Isotype Control Mouse IgG1-UNLB
Immunogen Murine Bax peptide (a.a. 3-16)
Specificity Mouse Bax
Alternalte Name(s) Apoptosis regulator BAX, BCL2 associated X protein
Description Bax is a 20-22 kDa member of the Bcl-2 family of proteins involved in regulation of programmed cell death, or apoptosis. In murine thymocytes it is expressed primarily as a cytoplasmic protein. Over-expression of Bax promotes apoptosis by formation of homodimers and through heterodimerization with Bcl-2, an inhibitor of apoptosis. The monoclonal antibody 5B7 recognizes both native and activated forms of Bax as well as Bax-Bcl-2 and Bax-Bcl-xL heterodimers.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Immunoblotting – Quality tested 1-3,5
Immunoprecipitation – Reported in literature 1,3,5
Flow Cytometry – Reported in literature 4
Immunocytochemistry – Reported in literature 4
Purification – Reported in literature 5
Recommended Dilutions Please refer to product specific Technical Bulletin
References
1. Hsu Y, Youle RJ. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 1997;272:13829-34. (Immunogen, WB, IP)
2. Hsu Y, Wolter KG, Youle RJ. Cytosol-to-membrane redistribution of Bax and Bcl-XL during apoptosis. Proc Natl Acad Sci USA. 1997;94:3668-72. (WB)
3. Havasi A, Li Z, Wang Z, Martin JL, Botla V, Ruchalski K, et al. Hsp27 inhibits Bax activation and apoptosis via a phosphatidylinositol 3-kinase-dependent mechanism. J Biol Chem. 2008;283:12305-13. (WB, IP)
4. Halicka HD, Smolewski P, Darzynkiewicz Z, Dai W, Traganos F. Arsenic trioxide arrests cells early in mitosis leading to apoptosis. Cell Cycle. 2002;1:200-8. (FC, ICC)
5. Hsu Y, Youle RJ. Bax in murine thymus is a soluble monomeric protein that displays differential detergent-induced conformations. J Biol Chem. 1998;273:10777-83. (WB, IP, Purification)
Back to Product List
Customer & Technical Service 800.722.2255 (USA & Canada) . 205.945.1774 . Fax 205.945.8768
Copyright © 2017 SouthernBiotech
Follow SouthernBiotech