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Mouse Anti-Cytokeratin 18-UNLB

Cat. No.
10085-01  
Size
0.1 mg  
Price (USD)
$185.00 
Qty
Add to Cart
Clone SB38b
Isotype Mouse (BALB/c) IgG2bκ
Isotype Control Mouse IgG2b-UNLB
Immunogen Recombinant N-terminal cytokeratin 18
Specificity Cytokeratin 18
Alternalte Name(s) CK 18, keratin 18
Description Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30% sequence homology. Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 18 is a type I intermediate filament protein that exists as a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 is expressed in all simple type epithelia and basal cells of many squamous and non-epidermal epithelia and is often co-expressed with cytokeratin 8. Tissues from the gastrointestinal tract, respiratory tract, and urogenital tract as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested
FLISA – Quality tested
Immunocytochemistry 1
Flow Cytometry 2
Immunoblotting 2
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01) followed by Donkey Anti-Mouse IgG(H+L), Multi-Species SP ads-AF555 (SB Cat. No. 6415-32) and DAPI.
  • Human carcinoma cell line HEp-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01) followed by Goat Anti-Mouse IgG2b, Human/Bovine/Horse SP ads-FITC (SB Cat. No. 1093-02) and DAPI.

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01) followed by Donkey Anti-Mouse IgG(H+L), Multi-Species SP ads-AF555 (SB Cat. No. 6415-32) and DAPI.
 

Human carcinoma cell line HEp-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01) followed by Goat Anti-Mouse IgG2b, Human/Bovine/Horse SP ads-FITC (SB Cat. No. 1093-02) and DAPI.
 
References
1. SouthernBiotech published data (ICC)
2. SouthernBiotech unpublished data (FC, WB)
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