Cat. No.
10112-08

Size
0.5 mg

Price (USD)
$275.00

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Technical Bulletin Certificate of
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Clone	BVD2-21C11
Isotype	Rat IgG_2aκ
Isotype Control	Rat IgG_2a-BIOT
Immunogen	E. coli-expressed human GM-CSF
Specificity	Human/Rhesus/Cynomolgus GM-CSF
Alternalte Name(s)	Granulocyte/macrophage colony-stimulating factor, CSF-α, pluripoietin-α, eosinophil colony stimulating factor, Eo-CSF, burst promoting activity, BPA
Format/Conjugate	BIOT (Biotin)
Buffer Formulation	Supplied in PBS/NaN₃
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	Store at 2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - ELISA-Detection – Quality tested ^1-7 ELISpot-Detection – Reported in literature ^4,8 Flow Cytometry – Reported in literature ^9,10 Immunohistochemistry-Frozen Sections – Reported in literature ^11,12 Immunohistochemistry – Reported in literature ¹³ Immunocytochemistry – Reported in literature ^14,15 Immunoprecipitation – Reported in literature ³ Neutralization – Reported in literature ^1,3 Multiplex-Detection– Reported in literature ¹⁶ Note – May be paired with the purified clone BVD2-23B6 (SB Cat. No. 10111-01) in a sandwich ELISA
Recommended Dilutions	Please refer to product specific Technical Bulletin

Standard curve generated with Rat Anti-Human GM-CSF-UNLB (SB Cat. No. 10111-01; Clone BVD2-23B6) and Rat Anti-Human GM-CSF-BIOT (SB Cat. No. 10112-08; Clone BVD2-21C11) followed by Streptavidin-HRP (SB Cat. No. 7100-05)

Standard curve generated with Rat Anti-Human GM-CSF-UNLB (SB Cat. No. 10111-01; Clone BVD2-23B6) and Rat Anti-Human GM-CSF-BIOT (SB Cat. No. 10112-08; Clone BVD2-21C11) followed by Streptavidin-HRP (SB Cat. No. 7100-05)

References

1.	Abrams JS, Silver JE, Van Dyke RE, Gleich GJ. Eosinophil-active cytokines in human disease: development and use of monoclonal antibodies to IL-3, IL-5 and GM-CSF. In: Gleich GJ, Kay AB, editors. Eosinophils in allergy and inflammation. New York: Marcel Dekker; 1994. p. 133-57. (Immunogen, ELISA-Detection, Neut)
2.	Bacchetta R, de Waal Malefijt R, Yssel H, Abrams J, de Vries JE, Spits H, et al. Host-reactive CD4⁺ and CD8⁺ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-γ and granulocyte/macrophage-colony-stimulating factor but not IL-4. J Immunol. 1990;144:902-8. (ELISA-Detection)
3.	Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, IP, Neut)
4.	Mahanty S, Abrams JS, King CL, Limaye AP, Nutman TB. Parallel regulation of IL-4 and IL-5 in human helminth infections. J Immunol. 1992;148:3567-71. (ELISA-Detection, ELISPOT-Detection)
5.	Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
6.	Mäkitalo B, Andersson M, Areström I, Karlén K, Villinger F, Ansari A, et al. ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques. J Immunol Methods. 2002;270:85-97. (ELISA-Detection, Rhesus & Cynomolgus Reactivity)
7.	Zhao W, Oskeritzian CA, Pozez AL, Schwartz LB. Cytokine production by skin-derived mast cells: endogenous proteases are responsible for degradation of cytokines. J Immunol. 2005;175:2635-42. (ELISA-Detection)
8.	Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Detection)
9.	Cousins DJ, Lee TH, Staynov DZ. Cytokine coexpression during human Th1/Th2 cell differentiation: direct evidence for coordinated expression of Th2 cytokines. J Immunol. 2002;169:2498-506. (FC)
10.	Raiden S, Schettini J, Salamone G, Trevani A, Vermeulen M, Gamberale R, et al. Human platelets produce granulocyte-macrophage colony-stimulating factor and delay eosinophil apoptosis. Lab Invest. 2003;83:589-98 (FC)
11.	Andersson J, Abrams J, Björk L, Funa K, Litton M, Ågren K, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994;83:16-24. (IHC-FS)
12.	Ulfgren A, Lindblad S, Klareskog L, Andersson J, Andersson U. Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis. Ann Rheum Dis. 1995;54:654-61. (IHC-FS)
13.	Saha S, Doe C, Mistry V, Siddiqui S, Parker D, Sleeman M, et al. Granulocyte-macrophage colony-stimulating factor expression in induced sputum and bronchial mucosa in asthma and COPD. Thorax. 2009;64:671-6. (IHC)
14.	Brauner A, Söderhäll M, Jacobson SH, Lundahl J, Andersson U, Andersson J. Escherichia coli-induced expression of IL-1α, IL-1β, IL-6 and IL-8 in normal human renal tubular epithelial cells. Clin Exp Immunol. 2001;124:423-8. (ICC)
15.	Lammel V, Stoeckle C, Padberg B, Zweifel R, Kienle DL, Reinhart WH, et al. Hypereosinophilia driven by GM-CSF in large-cell carcinoma of the lung. Lung Cancer. 2012;76:493-5. (ICC)
16.	Hutchinson KL, Villinger F, Miranda ME, Ksiazek TG, Peters CJ, Rollin PE. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6. (Multiplex-Detection, Cynomolgus Reactivity)

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