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Rat Anti-Human IL-5-BIOT

Cat. No.
10118-08  
Size
0.5 mg  
Price (USD)
$230.00 
Qty
Add to Cart
Clone JES1-5A10
Isotype Rat IgG2aκ
Isotype Control Mouse IgG2a-BIOT
Immunogen COS-expressed human IL-5
Specificity Human/Rhesus/Cynomolgus/Baboon/Sooty Mangabey IL-5
Alternalte Name(s) Interleukin-5, eosinophil differentiation factor, EDF, T cell replacing factor-1, TRF-1, eosinophil colony stimulating factor, Eo-CSF, B cell growth factor-2, BCGF-2, B cell differentiation factor for IgM, BCDF-m, IgA enhancing factor, IgA-EF
Format/Conjugate BIOT (Biotin)
Buffer Formulation Supplied in PBS/NaN3
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Capture – Quality tested
ELISA-Detection – Quality tested 1-10
ELISpot-Detection – Reported in literature 4,8,11-14
Flow Cytometry – Reported in literature 6
Neutralization – Reported in literature 1-3
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Standard curve generated with Rat Anti-Human IL-5-UNLB (SB Cat. No. 10118-01; Clone JES1-5A10) and Rat Anti-Human IL-5-BIOT (SB Cat. No. 10119-08; Clone JES1-39D10) followed by Streptavidin-HRP (SB Cat. No. 7100-05)

Standard curve generated with Rat Anti-Human IL-5-UNLB (SB Cat. No. 10118-01; Clone JES1-5A10) and Rat Anti-Human IL-5-BIOT (SB Cat. No. 10119-08; Clone JES1-39D10) followed by Streptavidin-HRP (SB Cat. No. 7100-05)
 
References
1. Abrams JS, Silver JE, Van Dyke RE, Gleich GJ. Eosinophil-active cytokines in human disease: development and use of monoclonal antibodies to IL-3, IL-5 and GM-CSF. In: Gleich GJ, Kay AB, editors. Eosinophils in allergy and inflammation. New York: Marcel Dekker; 1994. p. 133-57. (Immunogen, ELISA-Detection, Neut)
2. Bacchetta R, de Waal Malefijt R, Yssel H, Abrams J, de Vries JE, Spits H, et al. Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-γ and granulocyte/macrophage-colony-stimulating factor but not IL-4. J Immunol. 1990;144:902-8. (ELISA-Detection, Neut)
3. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut)
4. Mahanty S, Abrams JS, King CL, Limaye AP, Nutman TB. Parallel regulation of IL-4 and IL-5 in human helminth infections. J Immunol. 1992;148:3567-71. (ELISA-Detection, ELISPOT-Detection)
5. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
6. Krouwels FH, Nocker RE, Snoek M, Lutter R, van der Zee JS, Weller FR, et al. Immunocytochemical and flow cytofluorimetric detection of intracellular IL-4, IL-5 and IFN-γ: applications using blood- and airway-derived cells. J Immunol Methods. 1997;203:89-101. (ELISA-Detection, FC)
7. Nyindo M, Kariuki TM, Mola PW, Farah IO, Elson L, Blanton RE, et al. Role of adult worm antigen-specific immunoglobulin E in acquired immunity to Schistosoma mansoni infection in baboons. Infect Immun. 1999;67:636-42. (ELISA-Detection, Baboon Reactivity)
8. Mäkitalo B, Andersson M, Areström I, Karlén K, Villinger F, Ansari A, et al. ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques. J Immunol Methods. 2002;270:85-97. (ELISA-Detection, ELISPOT-Detection, Rhesus & Cynomolgus Reactivity)
9. Zhao W, Oskeritzian CA, Pozez AL, Schwartz LB. Cytokine production by skin-derived mast cells: endogenous proteases are responsible for degradation of cytokines. J Immunol. 2005;175:2635-42. (ELISA-Detection)
10. Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA. Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006;118:353-60. (ELISA-Detection)
11. Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, et al. Detection of low-frequency antigen-specific IL-10-producing CD4+ T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J Immunol Methods. 2003;279:111-21. (ELISPOT-Detection)
12. Wang Z, Metcalf B, Ribeiro RM, McClure H, Kaur A. Th-1-type cytotoxic CD8+ T-lymphocyte responses to simian immunodeficiency virus (SIV) are a consistent feature of natural SIV infection in sooty mangabeys. J Virol. 2006;80:2771-83. (ELISPOT-Detection, Sooty Mangabey Reactivity)
13. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Detection)
14. Pichyangkul S, Tongtawe P, Kum-Arb U, Yongvanitchit K, Gettayacamin M, Hollingdale MR, et al. Evaluation of the safety and immunogenicity of Plasmodium falciparum apical membrane antigen 1, merozoite surface protein 1 or RTS,S vaccines with adjuvant system AS02A administered alone or concurrently in rhesus monkeys. Vaccine. 2010;28:452-62. (ELISPOT-Detection, Rhesus Reactivity)
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