The Antibody Company - SouthernBiotech
 
   

Rat Anti-Mouse IL-6-BIOT

Cat. No.
10208-08  
Size
0.5 mg  
Price (USD)
$230.00 
Qty
Add to Cart
Clone MP5-32C11
Isotype Rat IgG2aκ
Isotype Control Mouse IgG2a-BIOT
Immunogen COS-expressed mouse IL-6
Specificity Mouse IL-6
Alternalte Name(s) Interleukin-6, hepatocyte stimulating factor, HSF, hybridoma/plasmacytoma growth factor, HPGFB cell stimulating factor-2, BSF-2, cytotoxic T cell differentiation factor, CDF
Format/Conjugate BIOT (Biotin)
Buffer Formulation Supplied in PBS/NaN3
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Detection – Quality tested 1-7
ELISpot-Detection – Reported in literature 4,8,9
Immunohistochemsitry-Frozen Sections – Reported in literature 10,11
Immunocytochemistry – Reported in literature 3
Multiplex-Detection – Reported in literature 5

Note – May be paired with the purified clone MP5-20F3 (SB Cat. No. 10207-01) in a sandwich ELISA
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Standard curve generated with Rat Anti-Mouse IL-6-UNLB (SB Cat. No. 10207-01; Clone MP5-20F3) and Rat Anti-Mouse IL-6-BIOT (SB Cat. No. 10208-08; Clone MP5-32C11) followed by Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05)

Standard curve generated with Rat Anti-Mouse IL-6-UNLB (SB Cat. No. 10207-01; Clone MP5-20F3) and Rat Anti-Mouse IL-6-BIOT (SB Cat. No. 10208-08; Clone MP5-32C11) followed by Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05)
 
References
1. Starnes HF Jr, Pearce MK, Tewari A, Yim JH, Zou J, Abrams JS. Anti-IL-6 monoclonal antibodies protect against lethal Escherichia coli infection and lethal tumor necrosis factor-α challenge in mice. J Immunol. 1990;145:4185-91. (ELISA-Detection)
2. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection)
3. Sander B, Höidén I, Andersson U, Möller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993;166:201-14. (ELISA-Detection, ICC)
4. Shirai A, Holmes K, Klinman D. Detection and quantitation of cells secreting IL-6 under physiologic conditions in BALB/c mice. J Immunol. 1993;150:793-9. (ELISA-Detection, ELISPOT-Detection)
5. Carson RT, Vignali DA. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods. 1999;227:41-52. (ELISA-Detection, Multiplex-Detection)
6. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
7. da Fonseca DM, Silva CL, Wowk PF, Paula MO, Ramos SG, Horn C, et al. Mycobacterium tuberculosis culture filtrate proteins plus CpG Oligodeoxynucleotides confer protection to Mycobacterium bovis BCG-primed mice by inhibiting interleukin-4 secretion. Infect Immun. 2009;77:5311-21. (ELISA-Detection)
8. Stern BV, Boehm BO, Tary-Lehmann M. Vaccination with tumor peptide in CpG adjuvant protects via IFN-γ-dependent CD4 cell immunity. J Immunol. 2002;168:6099-105. (ELISPOT-Detection)
9. Linker RA, Rott E, Hofstetter HH, Hanke T, Toyka KV, Gold R. EAE in beta-2 microglobulin-deficient mice: axonal damage is not dependent on MHC-I restricted immune responses. Neurobiol Dis. 2005;19:218-28. (ELISPOT-Detection)
10. Martinelli TM, Van Driel IR, Alderuccio F, Gleeson PA, Toh B. Analysis of mononuclear cell infiltrate and cytokine production in murine autoimmune gastritis. Gastroenterology. 1996;110:1791-802. (IHC-FS)
11. Hersmann GH, Kriegsmann J, Simon J, Hüttich C, Bräuer R. Expression of cell adhesion molecules and cytokines in murine antigen-induced arthritis. Cell Adhes Commun. 1998;6:69-82. (IHC-FS)
Back to Product List
Customer & Technical Service 800.722.2255 (USA & Canada) . 205.945.1774 . Fax 205.945.8768
Copyright © 2017 SouthernBiotech
Follow SouthernBiotech