Cat. No.
10214-08

Size
0.5 mg

Price (USD)
$275.00

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Technical Bulletin Certificate of
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Clone	TC11-8H4
Isotype	Rat IgG₁κ
Isotype Control	Rat IgG₁-BIOT
Immunogen	E. coli-expressed mouse IL-17
Specificity	Mouse IL-17A
Alternalte Name(s)	Interleukin-17A, cytotoxic T lymphocyte-associated antigen 8, CTLA-8
Format/Conjugate	BIOT (Biotin)
Buffer Formulation	Phosphate buffered saline containing 0.1% sodium azide
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - ELISA-Detection – Quality tested ¹ Flow Cytometry – Quality tested ⁵ ELISpot-Capture – Reported in literature ² ELISpot-Detection – Reported in literature ^3,4 Note – May be paired with the purified clone TC11-18H10 (SB Cat. No. 10215-01) in a sandwich ELISA
Recommended Dilutions	Please refer to product specific Technical Bulletin

PMA and ionomycin stimulated C57BL/6N mouse T lymphocyte EL-4 cell line was intracellularly stained with Rat Anti-Mouse IL-17A-FITC (SB Cat. No. 10214-02).

PMA and ionomycin stimulated C57BL/6N mouse T lymphocyte EL-4 cell line was intracellularly stained with Rat Anti-Mouse IL-17A-FITC (SB Cat. No. 10214-02).

References

1.	Amsen D, de Visser KE, Town T. Approaches to determine expression of inflammatory cytokines. Methods Mol Biol. 2009;511:107-42. (ELISA-Detection)
2.	Kish DD, Li X, Fairchild RL. CD8 T cells producing IL-17 and IFN-γ initiate the innate immune response required for responses to antigen skin challenge. J Immunol. 2009;182:5949-59. (ELISPOT-Capture)
3.	Faust SM, Lu G, Marini BL, Zou W, Gordon D, Iwakura Y, et al. Role of T cell TGFβ signaling and IL-17 in allograft acceptance and fibrosis associated with chronic rejection. J Immunol. 2009;183:7297-306. (ELISPOT-Detection)
4.	Nekrasova T, Shive C, Gao Y, Kawamura K, Guardia R, Landreth G, et al. ERK1-deficient mice show normal T cell effector function and are highly susceptible to experimental autoimmune encephalomyelitis. J Immunol. 2005;175:2374-80. (ELISPOT-Detection)
5.	Bending D, Newland S, Krejcí A, Phillips JM, Bray S, Cooke A. Epigenetic changes at Il12rb2 and Tbx21 in relation to plasticity behavior of Th17 cells. J Immunol. 2011;186:3373-82. (FC)

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Rat Anti-Mouse IL-17A-BIOT