Rat Anti-Mouse IL-17A-BIOT

Cat. No.
10214-08  
Size
0.5 mg   		Price (USD)
$275.00 
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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Clone TC11-8H4
Isotype Rat IgG1κ
Isotype Control Rat IgG1-BIOT
Immunogen E. coli-expressed mouse IL-17
Specificity Mouse IL-17A
Alternalte Name(s) Interleukin-17A, cytotoxic T lymphocyte-associated antigen 8, CTLA-8
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Detection – Quality tested 1
Flow Cytometry – Quality tested 5
ELISpot-Capture – Reported in literature 2
ELISpot-Detection – Reported in literature 3,4

Note – May be paired with the purified clone TC11-18H10 (SB Cat. No. 10215-01) in a sandwich ELISA
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794258
Gene ID
16171 (Mouse)
Gene Symbol
Il17a (Mouse)
Gene Aliases Ctla-8; Ctla8; IL-17; IL-17A; Il17
Uniprot ID
Q62386 (Mouse)
Uniprot Name
IL17_MOUSE 
  • Rat Anti-Mouse IL-17A-FITC
    PMA and ionomycin stimulated C57BL/6N mouse T lymphocyte EL-4 cell line was intracellularly stained with Rat Anti-Mouse IL-17A-FITC (SB Cat. No. 10214-02).

PMA and ionomycin stimulated C57BL/6N mouse T lymphocyte EL-4 cell line was intracellularly stained with Rat Anti-Mouse IL-17A-FITC (SB Cat. No. 10214-02).
 
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References
1. Amsen D, de Visser KE, Town T. Approaches to determine expression of inflammatory cytokines. Methods Mol Biol. 2009;511:107-42. (ELISA-Detection)
2. Kish DD, Li X, Fairchild RL. CD8 T cells producing IL-17 and IFN-γ initiate the innate immune response required for responses to antigen skin challenge. J Immunol. 2009;182:5949-59. (ELISPOT-Capture)
3. Faust SM, Lu G, Marini BL, Zou W, Gordon D, Iwakura Y, et al. Role of T cell TGFβ signaling and IL-17 in allograft acceptance and fibrosis associated with chronic rejection. J Immunol. 2009;183:7297-306. (ELISPOT-Detection)
4. Nekrasova T, Shive C, Gao Y, Kawamura K, Guardia R, Landreth G, et al. ERK1-deficient mice show normal T cell effector function and are highly susceptible to experimental autoimmune encephalomyelitis. J Immunol. 2005;175:2374-80. (ELISPOT-Detection)
5. Bending D, Newland S, Krejcí A, Phillips JM, Bray S, Cooke A. Epigenetic changes at Il12rb2 and Tbx21 in relation to plasticity behavior of Th17 cells. J Immunol. 2011;186:3373-82. (FC)